(R)‐α‐Trifluoromethylalanine as a 19F NMR Probe for the Monitoring of Protease Digestion of Peptides

Fluorinated non‐natural amino acids are useful tools for improving the bioavailability of peptides but can also serve as fluorinated probes in 19F NMR‐based enzymatic assays. We report herein that the use of the non‐natural α‐quaternarized (R)‐α‐trifluoromethylalanine ((R)‐α‐TfmAla) provides conveni...

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Veröffentlicht in:Chembiochem : a European journal of chemical biology 2022-01, Vol.23 (1), p.n/a
Hauptverfasser: Devillers, Emmanuelle, Chelain, Evelyne, Dalvit, Claudio, Brigaud, Thierry, Pytkowicz, Julien
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Sprache:eng
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Zusammenfassung:Fluorinated non‐natural amino acids are useful tools for improving the bioavailability of peptides but can also serve as fluorinated probes in 19F NMR‐based enzymatic assays. We report herein that the use of the non‐natural α‐quaternarized (R)‐α‐trifluoromethylalanine ((R)‐α‐TfmAla) provides convenient and accurate monitoring of trypsin proteolytic activity and increases resistance towards pepsin degradation. A useful fluorinated NMR probe: α‐Quaternarized (R)‐α‐trifluoromethylalanine (α‐TfmAla) is a good tool for providing convenient and accurate 19F NMR‐based monitoring of trypsin proteolytic activity and increases resistance towards pepsin degradation.
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.202100470