Investigation of genetic diversity and phylogenetic relationship of Ctenopharyngodon idella from different regions of Punjab by using SSR Markers

Genetic monitoring is a commonly used tool to find the genetic diversity, for this purpose, simple sequence repeat (SSR) markers is best implemented because of its polymorphic nature, low cost, variability and distribution. Ten SSR markers were used for the investigation of genetic variations in grass carp Ctenopharyngodon idella (C. idella). A total of 15 samples were collected from Khan Garh, Ghazi Ghatt and river Chenab Pakistan. Target loci were amplified and products were resolved on 10 % polyacrylamide gel. Mean value of polymorphism for all ten markers was 77.57%. Within 422 loci scored, 366 were found polymorphic in all samples. The allele number varied from 2 to 6 with average 3.70 values and allelic frequency ranged from 0.6777 to 0.9111with average value of 0.693. Gene diversity was found from 0.3011 to 0.5066 with average 0.3950 and PIC value ranged from 0.2366 to 0.4039 with average value of 0.2726. The values of Nei's genetic distant among populations of river Chenab were 0.1764, and 0.4411, while values of Jaccard's similarity coefficient were 0.7500 and 0.9167. These values showed that genetic makeup of C. idella from different regions of river Chenab varied from each other. Conclusion: The results showed that samples from same study area exhibited more genetic similarity but different from other areas indicating the genetic diversity of grass carp in different areas. It can be concluded that climatic variables, reproductive and geographic isolation influenced the genetic diversity in grass carp.