Micropropagation of Jasminanthes tuyetanhiae: an endemic and valuable herb in Vietnam
Jasminanthes tuyetanhiae is one of the precious herbs and good for human health, discovered in the Da-Bia Mountain (Phu Yen province, Vietnam). The identification of the CTW species (species believed to be J. tuyetanhiae collected in 2019) by molecular indicators showed that this species belongs to...
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Veröffentlicht in: | Plant cell, tissue and organ culture tissue and organ culture, 2022, Vol.148 (1), p.35-44 |
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Sprache: | eng |
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Zusammenfassung: | Jasminanthes tuyetanhiae
is one of the precious herbs and good for human health, discovered in the Da-Bia Mountain (Phu Yen province, Vietnam). The identification of the CTW species (species believed to be
J. tuyetanhiae
collected in 2019) by molecular indicators showed that this species belongs to the Apocynaceae family. High similarity (98.2%) in the
ITS1-5.8SrRNA-ITS2
gene sequence of CTW when compared on Genbank with
J. tuyetanhiae
and genetic distance difference (1.3%) - in the limit of difference of the same taxon considered on
mat
K sequence shows that CTW is likely to belong to this species. Surface sterilization of
ex vitro
explants with HgCl
2
(0.1%) for 3 min gave the highest sterilization efficiency compared to the remaining treatments with the induction explant rate of 93.33%. BA (0.5 mg l
−1
) is the most optimal for effective in vitro shoot multiplication in this species. Adding IBA to culture media at the appropriate concentration (0.5 mg l
−1
) increased rooting ability, shortened rooting time (13 days) and increased root length (6.53 mm). The ventilation culture system improves rooting efficiency, stimulates growth and induces secondary root formation—which is a suitable system for in vitro propagation of the
Jasminanthes tuyetanhiae
in this study.
Key message
Jasminanthes tuyetanhiae
is one of the precious herbs and good for human health, discovered in the Da Bia mountain of Phu Yen province. High similarity (98.2%) in the
ITS1-5.8SrRNA-ITS2
gene sequence of CTW when compared on Genbank with
Jaminanthes tuyetanhiae.
In vitro propagation is an effective method for the conservation and development of the
Jaminanthes tuyetanhiae
plantlets on a commercial scale. |
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ISSN: | 0167-6857 1573-5044 |
DOI: | 10.1007/s11240-021-02158-4 |