Influence of Mono- and Oligosaccharides on cbh1 Gene Transcription in the Filamentous Fungus Penicillium verruculosum
A method has been developed for the analysis of the induction of cbh1 gene transcription in the filamentous fungus Penicillium verruculosum after treatment with mono- and oligosaccharides. The method makes it possible to obtain mRNA, which encodes cellobiohydrolase 1 (CBH1), in an amount sufficient...
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| Veröffentlicht in: | Applied biochemistry and microbiology 2021-12, Vol.57 (9), p.925-932 |
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| container_issue | 9 |
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| container_title | Applied biochemistry and microbiology |
| container_volume | 57 |
| creator | Kislitsin, V. Yu Chulkin, A. M. Zorov, I. N. Shashkov, I. A. Satrutdinov, A. D. Sinitsyn, A. P. Rozhkova, A. M. |
| description | A method has been developed for the analysis of the induction of
cbh1
gene transcription in the filamentous fungus
Penicillium verruculosum
after treatment with mono- and oligosaccharides. The method makes it possible to obtain mRNA, which encodes cellobiohydrolase 1 (CBH1), in an amount sufficient for RT-qPCR. Citrate, a compound that does not cause carbon catabolite repression, was used as a carbon source. It was shown for the first time that xylose, gentiobiose, and, mainly, cellobiose induce the expression of the
cbh1
gene in
P. verruculosum
, while sophorose and a mixture of xylooligosaccharides with a degree of polymerization of 3–5 most likely serve as precursors of inducers. |
| doi_str_mv | 10.1134/S0003683821090040 |
| format | Article |
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cbh1
gene transcription in the filamentous fungus
Penicillium verruculosum
after treatment with mono- and oligosaccharides. The method makes it possible to obtain mRNA, which encodes cellobiohydrolase 1 (CBH1), in an amount sufficient for RT-qPCR. Citrate, a compound that does not cause carbon catabolite repression, was used as a carbon source. It was shown for the first time that xylose, gentiobiose, and, mainly, cellobiose induce the expression of the
cbh1
gene in
P. verruculosum
, while sophorose and a mixture of xylooligosaccharides with a degree of polymerization of 3–5 most likely serve as precursors of inducers.</description><identifier>ISSN: 0003-6838</identifier><identifier>EISSN: 1608-3024</identifier><identifier>DOI: 10.1134/S0003683821090040</identifier><language>eng</language><publisher>Moscow: Pleiades Publishing</publisher><subject>Biochemistry ; Biology ; Biomedical and Life Sciences ; Carbon ; Carbon sources ; Catabolite repression ; Cellobiohydrolase ; Cellobiose ; Citric acid ; Degree of polymerization ; Fungi ; Gene expression ; Genetic Engineering ; Gentiobiose ; Life Sciences ; Medical Microbiology ; Microbiology ; Oligosaccharides ; Penicillium ; Producers ; Selection ; Sophorose ; Transcription</subject><ispartof>Applied biochemistry and microbiology, 2021-12, Vol.57 (9), p.925-932</ispartof><rights>Pleiades Publishing, Inc. 2021. ISSN 0003-6838, Applied Biochemistry and Microbiology, 2021, Vol. 57, No. 9, pp. 925–932. © Pleiades Publishing, Inc., 2021. Russian Text © The Author(s), 2021, published in Biotekhnologiya, 2021, Vol. 37, No. 1, pp. 45–53.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-85ab66935829482f91ee3c022f07b191d18dea3f0cd04597646a2228ac6361ca3</citedby><cites>FETCH-LOGICAL-c316t-85ab66935829482f91ee3c022f07b191d18dea3f0cd04597646a2228ac6361ca3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S0003683821090040$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S0003683821090040$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Kislitsin, V. Yu</creatorcontrib><creatorcontrib>Chulkin, A. M.</creatorcontrib><creatorcontrib>Zorov, I. N.</creatorcontrib><creatorcontrib>Shashkov, I. A.</creatorcontrib><creatorcontrib>Satrutdinov, A. D.</creatorcontrib><creatorcontrib>Sinitsyn, A. P.</creatorcontrib><creatorcontrib>Rozhkova, A. M.</creatorcontrib><title>Influence of Mono- and Oligosaccharides on cbh1 Gene Transcription in the Filamentous Fungus Penicillium verruculosum</title><title>Applied biochemistry and microbiology</title><addtitle>Appl Biochem Microbiol</addtitle><description>A method has been developed for the analysis of the induction of
cbh1
gene transcription in the filamentous fungus
Penicillium verruculosum
after treatment with mono- and oligosaccharides. The method makes it possible to obtain mRNA, which encodes cellobiohydrolase 1 (CBH1), in an amount sufficient for RT-qPCR. Citrate, a compound that does not cause carbon catabolite repression, was used as a carbon source. It was shown for the first time that xylose, gentiobiose, and, mainly, cellobiose induce the expression of the
cbh1
gene in
P. verruculosum
, while sophorose and a mixture of xylooligosaccharides with a degree of polymerization of 3–5 most likely serve as precursors of inducers.</description><subject>Biochemistry</subject><subject>Biology</subject><subject>Biomedical and Life Sciences</subject><subject>Carbon</subject><subject>Carbon sources</subject><subject>Catabolite repression</subject><subject>Cellobiohydrolase</subject><subject>Cellobiose</subject><subject>Citric acid</subject><subject>Degree of polymerization</subject><subject>Fungi</subject><subject>Gene expression</subject><subject>Genetic Engineering</subject><subject>Gentiobiose</subject><subject>Life Sciences</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Oligosaccharides</subject><subject>Penicillium</subject><subject>Producers</subject><subject>Selection</subject><subject>Sophorose</subject><subject>Transcription</subject><issn>0003-6838</issn><issn>1608-3024</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp1UMtKAzEUDaJgrX6Au4Dr0Txm0mQpxdZCpYJ1PaSZO23KTFKTieDfm1LBhbg63HtecBC6peSeUl4-vBFCuJBcMkoUISU5QyMqiCw4YeU5Gh3p4shfoqsY9_lUQqoRSgvXdgmcAexb_OKdL7B2DV51duujNmang20gYu-w2ewonoMDvA7aRRPsYbD5bx0edoBnttM9uMGniGfJbTO8grPGdp1NPf6EEJJJnY-pv0YXre4i3PzgGL3PntbT52K5mi-mj8vCcCqGQlZ6I4TilWSqlKxVFIAbwlhLJhuqaENlA5q3xDSkrNRElEIzxqQ2ggtqNB-ju1PuIfiPBHGo9z4FlytrJmjeoGKiyip6UpngYwzQ1odgex2-akrq47r1n3Wzh508MWvdFsJv8v-mbxmve9o</recordid><startdate>20211201</startdate><enddate>20211201</enddate><creator>Kislitsin, V. 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Yu</creatorcontrib><creatorcontrib>Chulkin, A. M.</creatorcontrib><creatorcontrib>Zorov, I. N.</creatorcontrib><creatorcontrib>Shashkov, I. A.</creatorcontrib><creatorcontrib>Satrutdinov, A. D.</creatorcontrib><creatorcontrib>Sinitsyn, A. P.</creatorcontrib><creatorcontrib>Rozhkova, A. 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cbh1
gene transcription in the filamentous fungus
Penicillium verruculosum
after treatment with mono- and oligosaccharides. The method makes it possible to obtain mRNA, which encodes cellobiohydrolase 1 (CBH1), in an amount sufficient for RT-qPCR. Citrate, a compound that does not cause carbon catabolite repression, was used as a carbon source. It was shown for the first time that xylose, gentiobiose, and, mainly, cellobiose induce the expression of the
cbh1
gene in
P. verruculosum
, while sophorose and a mixture of xylooligosaccharides with a degree of polymerization of 3–5 most likely serve as precursors of inducers.</abstract><cop>Moscow</cop><pub>Pleiades Publishing</pub><doi>10.1134/S0003683821090040</doi><tpages>8</tpages></addata></record> |
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| ispartof | Applied biochemistry and microbiology, 2021-12, Vol.57 (9), p.925-932 |
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| source | SpringerLink_现刊 |
| subjects | Biochemistry Biology Biomedical and Life Sciences Carbon Carbon sources Catabolite repression Cellobiohydrolase Cellobiose Citric acid Degree of polymerization Fungi Gene expression Genetic Engineering Gentiobiose Life Sciences Medical Microbiology Microbiology Oligosaccharides Penicillium Producers Selection Sophorose Transcription |
| title | Influence of Mono- and Oligosaccharides on cbh1 Gene Transcription in the Filamentous Fungus Penicillium verruculosum |
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