Influence of Mono- and Oligosaccharides on cbh1 Gene Transcription in the Filamentous Fungus Penicillium verruculosum

A method has been developed for the analysis of the induction of cbh1 gene transcription in the filamentous fungus Penicillium verruculosum after treatment with mono- and oligosaccharides. The method makes it possible to obtain mRNA, which encodes cellobiohydrolase 1 (CBH1), in an amount sufficient...

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Veröffentlicht in:Applied biochemistry and microbiology 2021-12, Vol.57 (9), p.925-932
Hauptverfasser: Kislitsin, V. Yu, Chulkin, A. M., Zorov, I. N., Shashkov, I. A., Satrutdinov, A. D., Sinitsyn, A. P., Rozhkova, A. M.
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Sprache:eng
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Zusammenfassung:A method has been developed for the analysis of the induction of cbh1 gene transcription in the filamentous fungus Penicillium verruculosum after treatment with mono- and oligosaccharides. The method makes it possible to obtain mRNA, which encodes cellobiohydrolase 1 (CBH1), in an amount sufficient for RT-qPCR. Citrate, a compound that does not cause carbon catabolite repression, was used as a carbon source. It was shown for the first time that xylose, gentiobiose, and, mainly, cellobiose induce the expression of the cbh1 gene in P. verruculosum , while sophorose and a mixture of xylooligosaccharides with a degree of polymerization of 3–5 most likely serve as precursors of inducers.
ISSN:0003-6838
1608-3024
DOI:10.1134/S0003683821090040