Assessment of prenatal ovarian and serum estrogen concentration in Nili-Ravi buffalo fetus

The current study aims to assessment the estrogen concentration in both ovaries and serum in the fetus of the Nili Ravi buffalo. For this purpose, 73 female fetuses were collected from Sihala abattoir Islamabad, Pakistan. Both ovaries were obtained from fetuses with age ranging from day 51 to day 290 whereas; serum was available only for the fetuses ranging from the age of 146 days to 290 days. Radio immunoassay was used to measure the estrogen concentration and the data was analyzed through regression analysis of variance. Analysis of the data revealed mean ovarian estrogen concentration as 16.08±1.87 pg/mg of ovaries during the period of 51 to 290 days with peak value of 37.49±10.81pg/mg at 191-210 days. Ovarian estrogen concentration showed significant (P