Effect of salt stress on some physio-biochemical traits and antioxidative enzymes of two Brassica species under callus culture
The changes in lipid peroxidation, H 2 O 2 , proline, protein, involvement of different antioxidant systems (catalase, guaiacol peroxidase, ascorbate peroxidase) and callus-related traits were investigated under salt stress in the callus of two different ploidy levels of Brassica including B. juncea...
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Veröffentlicht in: | Plant cell, tissue and organ culture tissue and organ culture, 2021-12, Vol.147 (3), p.453-465 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | The changes in lipid peroxidation, H
2
O
2
, proline, protein, involvement of different antioxidant systems (catalase, guaiacol peroxidase, ascorbate peroxidase) and callus-related traits were investigated under salt stress in the callus of two different ploidy levels of
Brassica
including
B. juncea
and
B. oleracea
. The calluses of
B. juncea
genotypes were less sensitive to NaCl stress in comparison with those of
B. oleracea
while increasing the concentrations of NaCl from 0 to 200 mM. Tetraploid genotype (
B. juncea
cr3356) showed a significant increase in the contents of protein and proline, and guaiacol peroxidase activity and catalase enzymes at higher salinity levels. In addition, a significant decrease occurred in the amount of H
2
O
2
and malondialdehyde along with increasing the salinity intensity. Diploid cultivar (
B. oleracea
bra 2828) had the lowest enzymatic activities and the highest content of H
2
O
2
and malondialdehyde along with an increase in the salinity level. Therefore, this genotype was identified as the most sensitive cultivar under the salinity stress. The salinity resistance difference between diploid and amphidiploid species could be attributed to the differences in the ploidy level of these species. This result underlines the fact that the tetraploid genome of
B. oleracea
could be considered as a suitable candidate for production under salinity conditions through maintaining higher activities of antioxidant enzymes.
Key Message
This study revealed the effects of salinity stress on
Brassica juncea
and
B. oleracea
, through callus culture. In sum, these species showed different responses to the salinity stress. |
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ISSN: | 0167-6857 1573-5044 |
DOI: | 10.1007/s11240-021-02138-8 |