Expressions of miR-590 in Oral Lichen Planus and Oral Squamous Cell Carcinoma Tissues and Clinical Values

To detect the expression of miR-590 in oral lichen planus (OLP) tissues and oral squamous cell carcinoma (OSCC) tissues, and to analyze the correlations with clinicopathological characteristics and prognosis of OSCC patients. The oral mucosa tissues were selected from 180 patients with OLP or OSCC. They were divided into OLP group (n=92) and OSCC group (n=88), and 40 healthy volunteers with normal oral mucosa tissues were set as control group. Human tongue squamous cell carcinoma cell line SCC9 and human oral keratinocyte HOK were used. The expressions of miR-590 in tissues and cells were detected using qPCR. SCC9 cells were transfected with small interfering (si)-miR-590 and si-NC plasmids. MTT assay, flow cytometry and Transwell assay were used to detect cell proliferation, apoptosis, migration and invasion abilities, respectively. The expression levels of apoptosis-, migration- and invasion-related proteins were examined using Western blotting. Control, OLP and OSCC groups displayed successively increased expression of miR-590, suggesting that the expression was related to the TNM stage and lymph node metastasis of OSCC patients (P