ALLELIC POLYMORPHISM OF K-CASEIN GENE (CSN3) IN THREE MONTENEGRIN CATTLE BREEDS

The discovery of PCR-RFLP (Polimerase Chain Reaction - Restriction Fragment Lenght Polymorphism) generated renewed interest in the use of gene marker loci as an aid to selection programs and playing an increasingly important role as genetic markers in many fields of animal breeding. The studies of milk protein genes polymorphism and their application for the identification of individuals with desirable characteristics as well as for the selection and breeding programs is the future. First time it was imported on beginning of XX century as well as after Second World War, till the sixties, and used for improvement of production traits of Busha cattle. Amplification of 351 bp fragment of κ-casein was done using the polymerase chain reaction (PCR) with primers: forward (5'-TT TAT GGC CAT TCC ACC AA-3') and reverse (5'- ATT AGC CCA TTT CGC CTT CT-3') as reported by Dogru and Ozdemir (2009).