Effect of extraction solvents on total phenolic compound, lipid peroxidation, antioxidant and cytotoxic activity of leaves of Rubus armeniacus (Himalayan blackberry)

The objectives of this study were to determine effect of different extraction solvents on the total phenolic (TPC), flavonoid (TFC) and tannin contents (CTC) of Rubus armeniacus (Himalayan blackberry) dried leaves. Moreover, DPPH radical scavenging activity, reducing power, and β-carotene bleaching test were carried out to evaluate the antioxidant activity of the extracts. The oxidative stability of soybean oil (SBO) was also evaluated after addition of different extracts. The 80% methanolic and hexane extract of R. armeniacus had the highest and lowest content of TPC, TFC and CTC, respectively. The chlorogenic acid constituted the major component of the methanolic extract in HPLC analysis. It was observed that the 80% methanolic extract had the highest antioxidant activity. The oxidative stability of SBO was according to findings of antioxidant power of the extract. In conclusion, leaves of R. armeniacus can be used in food and pharmaceutical systems as a source of natural antioxidants.