Morpho-structural properties of ZnSe, TiO2-ZnSe materials and enzymatic activity of their bioinorganic hybrids with lysozyme

[Display omitted] •Micro-sized ZnSe and TiO2-ZnSe spheres were obtained by hydrothermal method.•TiO2 addition induces structural and physical–chemical changes to zinc selenide material.•ZnSe and TiO2-ZnSe surface properties were investigated before and after lysozyme adsorption.•TiO2-ZnSe proved higher lysozyme loading capacity than bare ZnSe.•The hydrolytic activity of Lys/ZnSe and Lys/TiO2-ZnSe is twofold higher than for free lysozyme. Structural and functional changes induced by TiO2 addition to spherical zinc selenide together with lysozyme adsorption lead to valuable bioinorganic catalysts with enhanced activity. Spherical zinc selenide and its composite with TiO2 were obtained by hydrothermal method and subsequently modified by lysozyme adsorption. Morphological, structural and functional characteristics of zinc selenide based materials (ZnSe, TiO2-ZnSe) and their hybrids with lysozyme (Lys/ZnSe, Lys/TiO2-ZnSe) were investigated. TiO2 addition to ZnSe results in significant changes of surface composition, electrochemical behavior and lysozyme retention capacity. The lysozyme modified ZnSe and TiO2-ZnSe surfaces doubles the biocatalysts efficiency for 4-Methylumbelliferyl β-D-N,N′,N″-triacetylchitotrioside hydrolysis reaction compared to free lysozyme.