Protein Stabilization Effect of Zwitterionic Osmolyte-bearing Polymer

Protein Stabilization Effect of Zwitterionic Osmolyte-bearing Polymer

Trimethylamine N-oxide (TMAO) protects proteins against denaturing stresses. In this study, a vinyl polymer with a TMAO side chain was synthesized and its effects on protein stabilization were evaluated. UV spectroscopy analysis showed that the polymer exhibited protein stabilization characteristics...

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Veröffentlicht in:Chemistry letters 2021-09, Vol.50 (9), p.1699-1702
Hauptverfasser: Anjum, Rubaiya, Nishimura, Shin-nosuke, Kobayashi, Shingo, Nishida, Kei, Anada, Takahisa, Tanaka, Masaru
Format: Artikel
Sprache:eng
Schlagworte:
Additives
Enzyme activity
Polymers
Proteins
Stabilization
Trimethylamine
Vinyl polymers
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Zusammenfassung:Trimethylamine N-oxide (TMAO) protects proteins against denaturing stresses. In this study, a vinyl polymer with a TMAO side chain was synthesized and its effects on protein stabilization were evaluated. UV spectroscopy analysis showed that the polymer exhibited protein stabilization characteristics. Furthermore, an enzyme activity assay indicated that the polymer showed a remarkable preservation effect at 4 °C compared to other commonly used additives. Therefore, TMAO-bearing polymer can potentially be used as a stabilizer for biochemical and pharmaceutical applications.
ISSN:0366-7022
1348-0715
DOI:10.1246/cl.210335