Synthesis and Study of Properties of Phosphinic Pseudo-Prolylglycylproline

Racemic phosphinic tripeptide 1 pyrrolidin-2-yl-{3-[(2-hydroxycarbonyl)-pyrrolidin-1-yl]-3-oxo-propyl}-phosphinic acid has been synthesized, its high resistance toward leucine aminopeptidase, carboxypeptidase Y, and the enzyme system of rat brain membranes has been shown. In vitro experiments with using Semax synthetic peptide have shown that the effect of tripeptide 1 on the hydrolysis rate of Semax in the case of leucine aminopeptidase and carboxypeptidase Y is minimal. In experiments using the enzyme system of rat brain membranes, the decrease of the rate of Semax hydrolysis has been more evident.