Molybdenum disulfide nanomaterial sensor-based detection of breast cancer microRNA

In this study, we sought to establish a rapid, simple, highly sensitive, and highly specific fluorescence sensor for the detection of breast cancer-related microRNA (miRNA) based on the novel nanomaterial known as molybdenum disulfide (MoS2). MoS2, a novel nanomaterial, was added to a fluorescently-labeled DNA probe system to construct a fluorescence sensor. Furthermore, non-complementary miRNAs, single-base mismatched miRNAs, and different concentrations of complementary miRNAs were hybridized with the fluorescently-labeled DNA probe. Then, miRNAs were detected by measuring changes in the fluorescence signals before and after hybridization. The detection was completed within 40 min. The detection limit of complementary miRNA reached pmol/L levels while distinguishing between complementary, non-complementary, and single-base mismatched miRNAs. The MoS2 fluorescence sensor demonstrated rapid analysis, high sensitivity, and high specificity. Additionally, this established detection method has the potential for application in the rapid detection of breast cancer-related miRNA as a promising cancer detection method.