Preparation and characterization azo dyes derived from 4- hydroxycoumarin and studying their analytical Applications

The preparation 0f azo dyes is the subject of this research. 4-hydroxycoumarin with each ofo-amino benzoic acid (3-[o-Amino benzoic acidazo]-4-hydroxycoumarin]) (B 1 ) and with Sulfa guanidine (3-[Sulfa guanidine azo]-4-hydroxycoumarin) (B 2 ) were syntheses. Azo compounds were characterized by I.R, CHN, 1 H-NMR Elctronic and Mass spectroscopy. At various pH levels, the electronic spectra of these azo dyes were investigated in terms of acid-base properties, which includes the determination of protonation and ionization constants as well as the establishment of isobestic points. On the electronic spectra, the solvent effect was also investigated at various polarities.