Visible light driven selective NADH regeneration using a system of water-soluble zinc porphyrin and homogeneous polymer-dispersed rhodium nanoparticles

We discovered the catalytic activity of Rh nanoparticles dispersed by polyvinylpyrrolidone (Rh-PVP) for NADH regeneration. The selective reduction of NAD + to enzyme active NADH has successfully been achieved in the presence of an electron donor, ZnTPPS as a photosensitizer, and Rh-PVP. Only 1,4-NADH was produced as the reduction product of NAD + , which was validated by an enzymatic assay and HPLC. We discovered the catalytic activity of Rh nanoparticles dispersed by polyvinylpyrrolidone for regioselective NAD + reduction to 1,4-NADH. Only 1,4-NADH was produced as the reduction product of NAD + with visible-light irradiation.