IDDF2021-ABS-0081 Detection of helicobacter pylori cagA, vacA, iceA virulence genes in patients with gastric cancer

IDDF2021-ABS-0081 Detection of helicobacter pylori cagA, vacA, iceA virulence genes in patients with gastric cancer

BackgroundHelicobacter pylori (H. pylori) infection causes chronic gastritis, peptic ulcer, gastric cancer and MALT-lymphoma. The association between genotypes of H. pylori and gastric cancer remains controversial. The aim of this study was: To investigate the H. pylori vacA alleles, iceA and cagA i...

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Veröffentlicht in:Gut 2021-09, Vol.70 (Suppl 2), p.A109-A111
Hauptverfasser: Viet, Hung Tran, Ngoc, Anh Tran, Quang, Duat Nguyen, Quang, Huy Duong, Hoang Thi Thu, Ha, Van, Phu Tran
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Sprache:eng
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Alleles
Biopsy
CagA protein
Chronic infection
Gastric cancer
Gastritis
Gene polymorphism
Helicobacter pylori
Lymphoma
Mucosal-associated lymphoid tissue
Peptic ulcers
Polymerase chain reaction
Urease
VacA protein
Virulence
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container_issue Suppl 2
container_start_page A109
container_title Gut
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creator Viet, Hung Tran
Ngoc, Anh Tran
Quang, Duat Nguyen
Quang, Huy Duong
Hoang Thi Thu, Ha
Van, Phu Tran
description BackgroundHelicobacter pylori (H. pylori) infection causes chronic gastritis, peptic ulcer, gastric cancer and MALT-lymphoma. The association between genotypes of H. pylori and gastric cancer remains controversial. The aim of this study was: To investigate the H. pylori vacA alleles, iceA and cagA in patients with gastric cancer.Methods91 patients with gastric cancer and 92 chronic gastritis were enrolled in this study. We obtained biopsy specimens from the stomach at the tumor margin in patients with gastric cancer, and from the antrum of stomach in patients with chronic gastritis. DNA extraction and polymerase chain reaction were used to detect the presence or absence of cagA, assess the polymorphism of vacA and iceA. Primers for PCR is presented in IDDF2021-ABS-0081 Table 1.ResultsA total of 183 patients (gastric cancer: 91 and chronic gastritis: 92) were found to have both positive urease test results and biopsy specimens from patients with chronic gastritis and gastric cancer. We found positive cagA (65/91, 71.4%) (IDDF2021-ABS-0081 Table 2), vacA (78/91, 85.7%) (IDDF2021-ABS-0081 Table 3), m1 (37/91, 40.7%), m2 (40/92, 44%), s1 (34/91, 37.4%), s2 (45/91, 49.5%) (IDDF2021-ABS-0081 Table 4), iceA (57/91,62.6%) (Table 7) and iceAl (50/91, 55%) (IDDF2021-ABS-0081 Table 5), iceA2 (10/91, 11%) (IDDF2021-ABS-0081 Table 6) strains in patients with gastric cancer. The odds of gastric cancer in H. pylori with iceA was 4.245 higher than those who did not carry iceA (IDDF2021-ABS-0081 Table 7). The odds of gastric cancer in H. pylori with iceA1 was 4.685 higher than those who did not carry iceA1 (IDDF2021-ABS-0081 Table 5).Abstract IDDF2021-ABS-0081 Table 1Primes for PCR Gene Primer designation Primer sequence Product (bp) Size of PCR Heat cycle CagA Cag5c-FCag3c-R 5’-GTTGATAACGCTGTCGCTTC-3’5’-GGGTTGTATGATATTTTCCATAA-3 350 94oC/3m VacA M1/m2 VAG-FVAG-R 5’-CAATCTGTCCAATCAAGCGAG-3’5’-GCGTCAAAATAATTCCAAGG-3’ 567/642 [94oC/1m, 55oC/1m, 72oC/1p] x 40 cycle72oC/10m VacA s1/s2 VAI-FVAI-R 5’-ATGGAAATACAACAAACACAC-3’5’-CTGCTTGAATGCGCCAAAC-3’ 259/286 iceA1F iceA1 R 5’-GRGRRRRRAACCAAAGTATC-3’5’-CTATAGCCASTYTCTTTGCA-3’ 247 iceA1/2 95oC/5m;[95oC/30s, 48C/45s, 72oC/45s]X35 cycle72oC/10m iceA2 F iceA2 R 5’-GTTGGGTATATCACAATTTAT-3’5’-TTRCCCTATTTTCTAGTAGGT-3’ 229 Abstract IDDF2021-ABS-0081 Table 2Rate of H. pylori CagA gene in the group of patients with gastric cancer and gastritis Group Gene Gastric cancer Gastritis OR 95% CI P Quantity (n) Rate (%) Quantity (n) Rate (%) CagA+
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The association between genotypes of H. pylori and gastric cancer remains controversial. The aim of this study was: To investigate the H. pylori vacA alleles, iceA and cagA in patients with gastric cancer.Methods91 patients with gastric cancer and 92 chronic gastritis were enrolled in this study. We obtained biopsy specimens from the stomach at the tumor margin in patients with gastric cancer, and from the antrum of stomach in patients with chronic gastritis. DNA extraction and polymerase chain reaction were used to detect the presence or absence of cagA, assess the polymorphism of vacA and iceA. Primers for PCR is presented in IDDF2021-ABS-0081 Table 1.ResultsA total of 183 patients (gastric cancer: 91 and chronic gastritis: 92) were found to have both positive urease test results and biopsy specimens from patients with chronic gastritis and gastric cancer. We found positive cagA (65/91, 71.4%) (IDDF2021-ABS-0081 Table 2), vacA (78/91, 85.7%) (IDDF2021-ABS-0081 Table 3), m1 (37/91, 40.7%), m2 (40/92, 44%), s1 (34/91, 37.4%), s2 (45/91, 49.5%) (IDDF2021-ABS-0081 Table 4), iceA (57/91,62.6%) (Table 7) and iceAl (50/91, 55%) (IDDF2021-ABS-0081 Table 5), iceA2 (10/91, 11%) (IDDF2021-ABS-0081 Table 6) strains in patients with gastric cancer. The odds of gastric cancer in H. pylori with iceA was 4.245 higher than those who did not carry iceA (IDDF2021-ABS-0081 Table 7). The odds of gastric cancer in H. pylori with iceA1 was 4.685 higher than those who did not carry iceA1 (IDDF2021-ABS-0081 Table 5).Abstract IDDF2021-ABS-0081 Table 1Primes for PCR Gene Primer designation Primer sequence Product (bp) Size of PCR Heat cycle CagA Cag5c-FCag3c-R 5’-GTTGATAACGCTGTCGCTTC-3’5’-GGGTTGTATGATATTTTCCATAA-3 350 94oC/3m VacA M1/m2 VAG-FVAG-R 5’-CAATCTGTCCAATCAAGCGAG-3’5’-GCGTCAAAATAATTCCAAGG-3’ 567/642 [94oC/1m, 55oC/1m, 72oC/1p] x 40 cycle72oC/10m VacA s1/s2 VAI-FVAI-R 5’-ATGGAAATACAACAAACACAC-3’5’-CTGCTTGAATGCGCCAAAC-3’ 259/286 iceA1F iceA1 R 5’-GRGRRRRRAACCAAAGTATC-3’5’-CTATAGCCASTYTCTTTGCA-3’ 247 iceA1/2 95oC/5m;[95oC/30s, 48C/45s, 72oC/45s]X35 cycle72oC/10m iceA2 F iceA2 R 5’-GTTGGGTATATCACAATTTAT-3’5’-TTRCCCTATTTTCTAGTAGGT-3’ 229 Abstract IDDF2021-ABS-0081 Table 2Rate of H. pylori CagA gene in the group of patients with gastric cancer and gastritis Group Gene Gastric cancer Gastritis OR 95% CI P Quantity (n) Rate (%) Quantity (n) Rate (%) CagA+ 65 71,4 43 46,7 2,8(1,5 – 5,2) 0,01 CagA- 26 28,6 49 53,3 Total 91 100 92 100 Abstract IDDF2021-ABS-0081 Table 3Rate of H. pylori vacA gene in the group of patients with gastric cancer and gastritis Group Gene Gastric cancer Gastritis OR 95% CI P Quantity (n) Rate (%) Quantity (n) Rate (%) VacA+ 78 85,7 46 50 6,0(2,9 – 12,2) &lt;0,05 VacA- 13 14,3 46 50 Total 91 100 92 100 Abstract IDDF2021-ABS-0081 Table 4The allele types of H. pylori with vacA gene in two groups. Gene VacA Gastric cancer Gastric cancer P Quantity (n) Rate (%) Quantity (n) Rate (%) s1 (+) (-) 3457 37,462,6 3260 34,865,2 0,716 s2 (+) (-) 4546 49,550,5 1874 19,680,4 0,0001 m1 (+) (-) 3754 40,759,3 3062 32,667,4 0,258 m2 (+) (-) 4051 44,056,0 1973 20,779,3 0,01 Abstract IDDF2021-ABS-0081 Table 5Relative risk of patients infected with H. pylori iceA genotype A1. Group IceA1 Gastric cancer (n,%) Gastritis (n,%) iceA1 (-) 41 (45.0) 73 (79.3) iceA1 (+) 50 (55.0) 19 (20.7) OR=4.685, 95%CI: [2.332-9.532]Abstract IDDF2021-ABS-0081 Table 6Relative risk of patients infected with H. pylori iceA genotype A2. Group IceA1 Gastric cancer (n,%) Gastritis (n,%) iceA2 (-) 81 (89.0) 85 (92.4) iceA2 (+) 10 (11.0) 7 (7.6) Abstract IDDF2021-ABS-0081 Table 7Relative risk of patients infected with H. pylori iceA +. Group IceA Gastric cancer (n,%) Gastritis (n,%) iceA (-) 34 (37.4) 66 (71.7) iceA (+) 57 (62.6) 26 (28.3) ConclusionsVacA s1, m2, and iceA are predominant in patients with gastric cancer. As compared with those in non-cancer patients, patients with gastric cancer have less vacA s1 and more m1 subtypes. The odds of gastric cancer in H. pylori with iceA was 4.245 higher than those who did not carry iceA. The odds of gastric cancer in H. pylori with iceA1 was 4.685 higher than those who did not carry iceA1.</description><identifier>ISSN: 0017-5749</identifier><identifier>EISSN: 1468-3288</identifier><identifier>DOI: 10.1136/gutjnl-2021-IDDF.129</identifier><language>eng</language><publisher>London: BMJ Publishing Group LTD</publisher><subject>Alleles ; Biopsy ; CagA protein ; Chronic infection ; Gastric cancer ; Gastritis ; Gene polymorphism ; Helicobacter pylori ; Lymphoma ; Mucosal-associated lymphoid tissue ; Peptic ulcers ; Polymerase chain reaction ; Urease ; VacA protein ; Virulence</subject><ispartof>Gut, 2021-09, Vol.70 (Suppl 2), p.A109-A111</ispartof><rights>Author(s) (or their employer(s)) 2021. No commercial re-use. See rights and permissions. Published by BMJ.</rights><rights>2021 Author(s) (or their employer(s)) 2021. No commercial re-use. See rights and permissions. Published by BMJ.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Viet, Hung Tran</creatorcontrib><creatorcontrib>Ngoc, Anh Tran</creatorcontrib><creatorcontrib>Quang, Duat Nguyen</creatorcontrib><creatorcontrib>Quang, Huy Duong</creatorcontrib><creatorcontrib>Hoang Thi Thu, Ha</creatorcontrib><creatorcontrib>Van, Phu Tran</creatorcontrib><title>IDDF2021-ABS-0081 Detection of helicobacter pylori cagA, vacA, iceA virulence genes in patients with gastric cancer</title><title>Gut</title><description>BackgroundHelicobacter pylori (H. pylori) infection causes chronic gastritis, peptic ulcer, gastric cancer and MALT-lymphoma. The association between genotypes of H. pylori and gastric cancer remains controversial. The aim of this study was: To investigate the H. pylori vacA alleles, iceA and cagA in patients with gastric cancer.Methods91 patients with gastric cancer and 92 chronic gastritis were enrolled in this study. We obtained biopsy specimens from the stomach at the tumor margin in patients with gastric cancer, and from the antrum of stomach in patients with chronic gastritis. DNA extraction and polymerase chain reaction were used to detect the presence or absence of cagA, assess the polymorphism of vacA and iceA. Primers for PCR is presented in IDDF2021-ABS-0081 Table 1.ResultsA total of 183 patients (gastric cancer: 91 and chronic gastritis: 92) were found to have both positive urease test results and biopsy specimens from patients with chronic gastritis and gastric cancer. We found positive cagA (65/91, 71.4%) (IDDF2021-ABS-0081 Table 2), vacA (78/91, 85.7%) (IDDF2021-ABS-0081 Table 3), m1 (37/91, 40.7%), m2 (40/92, 44%), s1 (34/91, 37.4%), s2 (45/91, 49.5%) (IDDF2021-ABS-0081 Table 4), iceA (57/91,62.6%) (Table 7) and iceAl (50/91, 55%) (IDDF2021-ABS-0081 Table 5), iceA2 (10/91, 11%) (IDDF2021-ABS-0081 Table 6) strains in patients with gastric cancer. The odds of gastric cancer in H. pylori with iceA was 4.245 higher than those who did not carry iceA (IDDF2021-ABS-0081 Table 7). The odds of gastric cancer in H. pylori with iceA1 was 4.685 higher than those who did not carry iceA1 (IDDF2021-ABS-0081 Table 5).Abstract IDDF2021-ABS-0081 Table 1Primes for PCR Gene Primer designation Primer sequence Product (bp) Size of PCR Heat cycle CagA Cag5c-FCag3c-R 5’-GTTGATAACGCTGTCGCTTC-3’5’-GGGTTGTATGATATTTTCCATAA-3 350 94oC/3m VacA M1/m2 VAG-FVAG-R 5’-CAATCTGTCCAATCAAGCGAG-3’5’-GCGTCAAAATAATTCCAAGG-3’ 567/642 [94oC/1m, 55oC/1m, 72oC/1p] x 40 cycle72oC/10m VacA s1/s2 VAI-FVAI-R 5’-ATGGAAATACAACAAACACAC-3’5’-CTGCTTGAATGCGCCAAAC-3’ 259/286 iceA1F iceA1 R 5’-GRGRRRRRAACCAAAGTATC-3’5’-CTATAGCCASTYTCTTTGCA-3’ 247 iceA1/2 95oC/5m;[95oC/30s, 48C/45s, 72oC/45s]X35 cycle72oC/10m iceA2 F iceA2 R 5’-GTTGGGTATATCACAATTTAT-3’5’-TTRCCCTATTTTCTAGTAGGT-3’ 229 Abstract IDDF2021-ABS-0081 Table 2Rate of H. pylori CagA gene in the group of patients with gastric cancer and gastritis Group Gene Gastric cancer Gastritis OR 95% CI P Quantity (n) Rate (%) Quantity (n) Rate (%) CagA+ 65 71,4 43 46,7 2,8(1,5 – 5,2) 0,01 CagA- 26 28,6 49 53,3 Total 91 100 92 100 Abstract IDDF2021-ABS-0081 Table 3Rate of H. pylori vacA gene in the group of patients with gastric cancer and gastritis Group Gene Gastric cancer Gastritis OR 95% CI P Quantity (n) Rate (%) Quantity (n) Rate (%) VacA+ 78 85,7 46 50 6,0(2,9 – 12,2) &lt;0,05 VacA- 13 14,3 46 50 Total 91 100 92 100 Abstract IDDF2021-ABS-0081 Table 4The allele types of H. pylori with vacA gene in two groups. Gene VacA Gastric cancer Gastric cancer P Quantity (n) Rate (%) Quantity (n) Rate (%) s1 (+) (-) 3457 37,462,6 3260 34,865,2 0,716 s2 (+) (-) 4546 49,550,5 1874 19,680,4 0,0001 m1 (+) (-) 3754 40,759,3 3062 32,667,4 0,258 m2 (+) (-) 4051 44,056,0 1973 20,779,3 0,01 Abstract IDDF2021-ABS-0081 Table 5Relative risk of patients infected with H. pylori iceA genotype A1. Group IceA1 Gastric cancer (n,%) Gastritis (n,%) iceA1 (-) 41 (45.0) 73 (79.3) iceA1 (+) 50 (55.0) 19 (20.7) OR=4.685, 95%CI: [2.332-9.532]Abstract IDDF2021-ABS-0081 Table 6Relative risk of patients infected with H. pylori iceA genotype A2. Group IceA1 Gastric cancer (n,%) Gastritis (n,%) iceA2 (-) 81 (89.0) 85 (92.4) iceA2 (+) 10 (11.0) 7 (7.6) Abstract IDDF2021-ABS-0081 Table 7Relative risk of patients infected with H. pylori iceA +. Group IceA Gastric cancer (n,%) Gastritis (n,%) iceA (-) 34 (37.4) 66 (71.7) iceA (+) 57 (62.6) 26 (28.3) ConclusionsVacA s1, m2, and iceA are predominant in patients with gastric cancer. As compared with those in non-cancer patients, patients with gastric cancer have less vacA s1 and more m1 subtypes. The odds of gastric cancer in H. pylori with iceA was 4.245 higher than those who did not carry iceA. The odds of gastric cancer in H. pylori with iceA1 was 4.685 higher than those who did not carry iceA1.</description><subject>Alleles</subject><subject>Biopsy</subject><subject>CagA protein</subject><subject>Chronic infection</subject><subject>Gastric cancer</subject><subject>Gastritis</subject><subject>Gene polymorphism</subject><subject>Helicobacter pylori</subject><subject>Lymphoma</subject><subject>Mucosal-associated lymphoid tissue</subject><subject>Peptic ulcers</subject><subject>Polymerase chain reaction</subject><subject>Urease</subject><subject>VacA protein</subject><subject>Virulence</subject><issn>0017-5749</issn><issn>1468-3288</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNotkLFOwzAQhi0EEqXwBgyWWElrO3Ycj6WlUKkSAzBbseOkjtIkOE5RNxZelCfBoSw-yff9d6cPgFuMZhjHybwcfNXUEUEER5vVaj3DRJyBCaZJGsUkTc_BBCHMI8apuARXfV8hhNJU4AkYRv4vuHh4jcIv_vn6XhlvtLdtA9sC7kxtdasy7Y2D3bFunYU6Kxf38JDp8FptFvBg3VCbRhtYmsb00Dawy7w1je_hp_U7WGa9d1aHZIDcNbgosro3N_91Ct7Xj2_L52j78rRZLraRwpiKSBGSESyKhJg8NznHnCqRM8PC8QU2WjBKiYp1EdpMKJWLhFIc85hzxtM8jqfg7jS3c-3HYHovq3ZwTVgpCUtSykQwFaj5iVL7SnbO7jN3lBjJ0a08uZWjIjm6kmPiFwwpbco</recordid><startdate>202109</startdate><enddate>202109</enddate><creator>Viet, Hung Tran</creator><creator>Ngoc, Anh Tran</creator><creator>Quang, Duat Nguyen</creator><creator>Quang, Huy Duong</creator><creator>Hoang Thi Thu, Ha</creator><creator>Van, Phu Tran</creator><general>BMJ Publishing Group LTD</general><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BTHHO</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope></search><sort><creationdate>202109</creationdate><title>IDDF2021-ABS-0081 Detection of helicobacter pylori cagA, vacA, iceA virulence genes in patients with gastric cancer</title><author>Viet, Hung Tran ; Ngoc, Anh Tran ; Quang, Duat Nguyen ; Quang, Huy Duong ; Hoang Thi Thu, Ha ; Van, Phu Tran</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b1149-b22a219f62edded7174b9d5e5088f1ec95442b3cfedd59bbd9644137377578d33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Alleles</topic><topic>Biopsy</topic><topic>CagA protein</topic><topic>Chronic infection</topic><topic>Gastric cancer</topic><topic>Gastritis</topic><topic>Gene polymorphism</topic><topic>Helicobacter pylori</topic><topic>Lymphoma</topic><topic>Mucosal-associated lymphoid tissue</topic><topic>Peptic ulcers</topic><topic>Polymerase chain reaction</topic><topic>Urease</topic><topic>VacA protein</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Viet, Hung Tran</creatorcontrib><creatorcontrib>Ngoc, Anh Tran</creatorcontrib><creatorcontrib>Quang, Duat Nguyen</creatorcontrib><creatorcontrib>Quang, Huy Duong</creatorcontrib><creatorcontrib>Hoang Thi Thu, Ha</creatorcontrib><creatorcontrib>Van, Phu Tran</creatorcontrib><collection>ProQuest Central (Corporate)</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>BMJ Journals</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>ProQuest Health &amp; Medical Research Collection</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Health &amp; Nursing</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Applied &amp; Life Sciences</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><jtitle>Gut</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Viet, Hung Tran</au><au>Ngoc, Anh Tran</au><au>Quang, Duat Nguyen</au><au>Quang, Huy Duong</au><au>Hoang Thi Thu, Ha</au><au>Van, Phu Tran</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>IDDF2021-ABS-0081 Detection of helicobacter pylori cagA, vacA, iceA virulence genes in patients with gastric cancer</atitle><jtitle>Gut</jtitle><date>2021-09</date><risdate>2021</risdate><volume>70</volume><issue>Suppl 2</issue><spage>A109</spage><epage>A111</epage><pages>A109-A111</pages><issn>0017-5749</issn><eissn>1468-3288</eissn><abstract>BackgroundHelicobacter pylori (H. pylori) infection causes chronic gastritis, peptic ulcer, gastric cancer and MALT-lymphoma. The association between genotypes of H. pylori and gastric cancer remains controversial. The aim of this study was: To investigate the H. pylori vacA alleles, iceA and cagA in patients with gastric cancer.Methods91 patients with gastric cancer and 92 chronic gastritis were enrolled in this study. We obtained biopsy specimens from the stomach at the tumor margin in patients with gastric cancer, and from the antrum of stomach in patients with chronic gastritis. DNA extraction and polymerase chain reaction were used to detect the presence or absence of cagA, assess the polymorphism of vacA and iceA. Primers for PCR is presented in IDDF2021-ABS-0081 Table 1.ResultsA total of 183 patients (gastric cancer: 91 and chronic gastritis: 92) were found to have both positive urease test results and biopsy specimens from patients with chronic gastritis and gastric cancer. We found positive cagA (65/91, 71.4%) (IDDF2021-ABS-0081 Table 2), vacA (78/91, 85.7%) (IDDF2021-ABS-0081 Table 3), m1 (37/91, 40.7%), m2 (40/92, 44%), s1 (34/91, 37.4%), s2 (45/91, 49.5%) (IDDF2021-ABS-0081 Table 4), iceA (57/91,62.6%) (Table 7) and iceAl (50/91, 55%) (IDDF2021-ABS-0081 Table 5), iceA2 (10/91, 11%) (IDDF2021-ABS-0081 Table 6) strains in patients with gastric cancer. The odds of gastric cancer in H. pylori with iceA was 4.245 higher than those who did not carry iceA (IDDF2021-ABS-0081 Table 7). The odds of gastric cancer in H. pylori with iceA1 was 4.685 higher than those who did not carry iceA1 (IDDF2021-ABS-0081 Table 5).Abstract IDDF2021-ABS-0081 Table 1Primes for PCR Gene Primer designation Primer sequence Product (bp) Size of PCR Heat cycle CagA Cag5c-FCag3c-R 5’-GTTGATAACGCTGTCGCTTC-3’5’-GGGTTGTATGATATTTTCCATAA-3 350 94oC/3m VacA M1/m2 VAG-FVAG-R 5’-CAATCTGTCCAATCAAGCGAG-3’5’-GCGTCAAAATAATTCCAAGG-3’ 567/642 [94oC/1m, 55oC/1m, 72oC/1p] x 40 cycle72oC/10m VacA s1/s2 VAI-FVAI-R 5’-ATGGAAATACAACAAACACAC-3’5’-CTGCTTGAATGCGCCAAAC-3’ 259/286 iceA1F iceA1 R 5’-GRGRRRRRAACCAAAGTATC-3’5’-CTATAGCCASTYTCTTTGCA-3’ 247 iceA1/2 95oC/5m;[95oC/30s, 48C/45s, 72oC/45s]X35 cycle72oC/10m iceA2 F iceA2 R 5’-GTTGGGTATATCACAATTTAT-3’5’-TTRCCCTATTTTCTAGTAGGT-3’ 229 Abstract IDDF2021-ABS-0081 Table 2Rate of H. pylori CagA gene in the group of patients with gastric cancer and gastritis Group Gene Gastric cancer Gastritis OR 95% CI P Quantity (n) Rate (%) Quantity (n) Rate (%) CagA+ 65 71,4 43 46,7 2,8(1,5 – 5,2) 0,01 CagA- 26 28,6 49 53,3 Total 91 100 92 100 Abstract IDDF2021-ABS-0081 Table 3Rate of H. pylori vacA gene in the group of patients with gastric cancer and gastritis Group Gene Gastric cancer Gastritis OR 95% CI P Quantity (n) Rate (%) Quantity (n) Rate (%) VacA+ 78 85,7 46 50 6,0(2,9 – 12,2) &lt;0,05 VacA- 13 14,3 46 50 Total 91 100 92 100 Abstract IDDF2021-ABS-0081 Table 4The allele types of H. pylori with vacA gene in two groups. Gene VacA Gastric cancer Gastric cancer P Quantity (n) Rate (%) Quantity (n) Rate (%) s1 (+) (-) 3457 37,462,6 3260 34,865,2 0,716 s2 (+) (-) 4546 49,550,5 1874 19,680,4 0,0001 m1 (+) (-) 3754 40,759,3 3062 32,667,4 0,258 m2 (+) (-) 4051 44,056,0 1973 20,779,3 0,01 Abstract IDDF2021-ABS-0081 Table 5Relative risk of patients infected with H. pylori iceA genotype A1. Group IceA1 Gastric cancer (n,%) Gastritis (n,%) iceA1 (-) 41 (45.0) 73 (79.3) iceA1 (+) 50 (55.0) 19 (20.7) OR=4.685, 95%CI: [2.332-9.532]Abstract IDDF2021-ABS-0081 Table 6Relative risk of patients infected with H. pylori iceA genotype A2. Group IceA1 Gastric cancer (n,%) Gastritis (n,%) iceA2 (-) 81 (89.0) 85 (92.4) iceA2 (+) 10 (11.0) 7 (7.6) Abstract IDDF2021-ABS-0081 Table 7Relative risk of patients infected with H. pylori iceA +. Group IceA Gastric cancer (n,%) Gastritis (n,%) iceA (-) 34 (37.4) 66 (71.7) iceA (+) 57 (62.6) 26 (28.3) ConclusionsVacA s1, m2, and iceA are predominant in patients with gastric cancer. As compared with those in non-cancer patients, patients with gastric cancer have less vacA s1 and more m1 subtypes. The odds of gastric cancer in H. pylori with iceA was 4.245 higher than those who did not carry iceA. The odds of gastric cancer in H. pylori with iceA1 was 4.685 higher than those who did not carry iceA1.</abstract><cop>London</cop><pub>BMJ Publishing Group LTD</pub><doi>10.1136/gutjnl-2021-IDDF.129</doi><oa>free_for_read</oa></addata></record>
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ispartof Gut, 2021-09, Vol.70 (Suppl 2), p.A109-A111
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source PubMed Central
subjects Alleles
Biopsy
CagA protein
Chronic infection
Gastric cancer
Gastritis
Gene polymorphism
Helicobacter pylori
Lymphoma
Mucosal-associated lymphoid tissue
Peptic ulcers
Polymerase chain reaction
Urease
VacA protein
Virulence
title IDDF2021-ABS-0081 Detection of helicobacter pylori cagA, vacA, iceA virulence genes in patients with gastric cancer
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