IDDF2021-ABS-0081 Detection of helicobacter pylori cagA, vacA, iceA virulence genes in patients with gastric cancer

BackgroundHelicobacter pylori (H. pylori) infection causes chronic gastritis, peptic ulcer, gastric cancer and MALT-lymphoma. The association between genotypes of H. pylori and gastric cancer remains controversial. The aim of this study was: To investigate the H. pylori vacA alleles, iceA and cagA in patients with gastric cancer.Methods91 patients with gastric cancer and 92 chronic gastritis were enrolled in this study. We obtained biopsy specimens from the stomach at the tumor margin in patients with gastric cancer, and from the antrum of stomach in patients with chronic gastritis. DNA extraction and polymerase chain reaction were used to detect the presence or absence of cagA, assess the polymorphism of vacA and iceA. Primers for PCR is presented in IDDF2021-ABS-0081 Table 1.ResultsA total of 183 patients (gastric cancer: 91 and chronic gastritis: 92) were found to have both positive urease test results and biopsy specimens from patients with chronic gastritis and gastric cancer. We found positive cagA (65/91, 71.4%) (IDDF2021-ABS-0081 Table 2), vacA (78/91, 85.7%) (IDDF2021-ABS-0081 Table 3), m1 (37/91, 40.7%), m2 (40/92, 44%), s1 (34/91, 37.4%), s2 (45/91, 49.5%) (IDDF2021-ABS-0081 Table 4), iceA (57/91,62.6%) (Table 7) and iceAl (50/91, 55%) (IDDF2021-ABS-0081 Table 5), iceA2 (10/91, 11%) (IDDF2021-ABS-0081 Table 6) strains in patients with gastric cancer. The odds of gastric cancer in H. pylori with iceA was 4.245 higher than those who did not carry iceA (IDDF2021-ABS-0081 Table 7). The odds of gastric cancer in H. pylori with iceA1 was 4.685 higher than those who did not carry iceA1 (IDDF2021-ABS-0081 Table 5).Abstract IDDF2021-ABS-0081 Table 1Primes for PCR Gene Primer designation Primer sequence Product (bp) Size of PCR Heat cycle CagA Cag5c-FCag3c-R 5’-GTTGATAACGCTGTCGCTTC-3’5’-GGGTTGTATGATATTTTCCATAA-3 350 94oC/3m VacA M1/m2 VAG-FVAG-R 5’-CAATCTGTCCAATCAAGCGAG-3’5’-GCGTCAAAATAATTCCAAGG-3’ 567/642 [94oC/1m, 55oC/1m, 72oC/1p] x 40 cycle72oC/10m VacA s1/s2 VAI-FVAI-R 5’-ATGGAAATACAACAAACACAC-3’5’-CTGCTTGAATGCGCCAAAC-3’ 259/286 iceA1F iceA1 R 5’-GRGRRRRRAACCAAAGTATC-3’5’-CTATAGCCASTYTCTTTGCA-3’ 247 iceA1/2 95oC/5m;[95oC/30s, 48C/45s, 72oC/45s]X35 cycle72oC/10m iceA2 F iceA2 R 5’-GTTGGGTATATCACAATTTAT-3’5’-TTRCCCTATTTTCTAGTAGGT-3’ 229 Abstract IDDF2021-ABS-0081 Table 2Rate of H. pylori CagA gene in the group of patients with gastric cancer and gastritis Group Gene Gastric cancer Gastritis OR 95% CI P Quantity (n) Rate (%) Quantity (n) Rate (%) CagA+