Research of component composition of mineralized bone implants by Raman spectroscopy

The results of Raman spectroscopy to evaluate alternative sources for donor bone implants using the "Lioplast" technology are presented. It is shown that Raman spectroscopy can be used to assess the component composition of the surface of bone implants during their treatment. It has been found that major differences were at Raman bands 1448, 1735 (lipids & fatty acid), 850 and 875 cm-1 (proline & hydroxyproline), 1001 and 1026 cm-1 (phenylalanine) and 1272, 1560 cm-1 (amide II, amide III). The criteria which allow to evaluate the component composition in the production of bioimplants are proposed, and a two-dimensional analysis was carried out. The analysis showed that the main components of the extracellular matrix necessary for the implementation of osteoinductive and osteoconductive properties of biomaterials are saved during processing. Lipids were removed that improved the quality of the material, providing the possibility of a good clinical effect.