NMR reveals the interplay between SilE and SilB model peptides in the context of silver resistance

SilE and SilB are both proteins involved in the silver efflux pump found in Gram-negative bacteria such as S. typhimurium . Using model peptides along with NMR and CD experiments, we show how SilE may store silver ions prior to delivery and we hypothesize for the first time the interplay between SilB and SilE. Illustrative sketch representative of the interplay between the SilB and SilE proteins in the context of silver bacteria resistance. In case of an excess of silver, SilE plays the role of a regulator and avoids a collapse of the silver efflux pump.