1194-P: Promotive Effects of Recombinant Human Adiponectin on Bone Regeneration by Paracrine

Nonunion and delayed fracture healing remains a great challenge in nowadays clinical practice. Bone and fat hold a close relationship to remain balanced through hormones and cytokines. Herein, we provided a facile and efficient method to obtain high purity and high yield recombinant human adiponectin (ADPN). The biocompatibility and the pharmaceutical behaviors were evaluated in the Sprague Dawley rats. In rat tibia fracture model, significantly accelerated bone healing was observed; the indices of osteogenesis enhanced at the fracture site, but not in blood. The underlying mechanism was primarily assessed and the expression of bone morphogenic protein 2 (BMP-2) and the adiponectin receptor 1 (AdipoR1) showed markedly increase at the fracture site with linear relationship. Cell experiments confirmed that the expression of BMP-2 and osteogenesis markers increased obviously in ADPN treatment groups; but reduced significantly when the expression of AdipoR1 was inhibited by SiRNA; and the differentiation of osteoblasts and calcification were significantly delayed after AdipoR1 SiRNA. Our study provided a feasible and efficacious way for bone fracture treatment with local administration of ADPN.