Bactericidal Activity of Zinc Oxide Nanoparticles

The present study was aimed to evaluate the antibacterial activity of zinc oxide nanoparticles against the pathogen isolated from diabetic foot ulcer. The isolate was identified by MALDI-TOF. The susceptibility of the isolate to antibiotic was evaluated by disc diffusion assay. Chemically synthesized zinc oxide nano particle was characterized for its size distribution by dynamic light scattering, morphology by FESEM and elemental composition by energy dispersive X-rayspectroscopy. The wound isolate was identified asE. coli. Thestrain wasresistant to β lactam and aminoglycoside antibiotics. The size of zinc oxide nano particle was recorded as 55nm. FESEM revealed the spherical shape of zinc oxide nanoparticles. UV visible spectrophotometry confirms the presence of zinc oxide nanoparticles with an absorbance maximum at 372 nm. Zinc oxide nanoparticles inhibited E. coli with an inhibition zone of 25 mm.Thus, thestudy provedthe-bacterial activity of zinc oxide nanoparticles against multi drug resistant E. coli.