IS THERE AN EPIGENETIC SIGNATURE ASSOCIATED WITH ADIPOSITY? LONGITUDINAL RESULTS IN RELATION TO TWO WEIGHT LOSS STRATEGIES

Introduction: Studies have shown that weight loss through interventions such as exercise, low calorie diet and bariatric surgery can modulate the dynamics and reverse methylation levels in different human tissues, providing the search for target markers to treat obesity-related comorbidities. Objectives: This study aimed to evaluate whether different treatments for obesity are able to modify epigenetic signature related to adiposity. Methods: 35 obese women were assessed before and after 6-week of hypocaloric dietary intervention (n=11, age: 36.9±10.3 years; BMI: 58.5±10.5kg/m2) or before and after 6months of gastric bypass (n=24, age: 36.9±10.2 years; BMI: 43.3±5.7kg/m2). These participants were compared with sexand age-matched normal-weight women (BMI: 22.5±1.6kg/m2). Anthropometric and body composition evaluation were performed in each time. Genome-wide DNA methylation analysis was performed in DNA extracted from peripheral blood leukocytes using Infinium Human Methylation 450 BeadChip assay. Changes (Δβ) in methylation level of each CpGs were calculated. A threshold for the significant CpG sites based on Δβ with a minimum value of 10%, p