HYDROLYZED PROTEINS OF PEA (PISUM SATIVUM) AND RICE (ORYZA SATIVA) AND ITS EFFECT ON ADIPOGENESIS IN THE 3T3-L1 CELL LINE

Introduction: At least 50% of the population has an excess of fat mass, which is closely related to the development of chronic degenerative diseases. Recently, scientific information put proteins, mainly plant proteins, as bioactive compounds. Pea (Pisum sativum) and rice (Oryza sativa) proteins have technological and nutritional functions; however, there is little information about its impact on adipose tissue. Objectives: Evaluate the effect of pea protein (Pisum sativum) and rice (Oryza sativa) hydrolysates on adipogenesis on the 3T3-L1 cell line. Methods: Protein isolates a)pea and b)rice were hydrolyzed by an in vitro digestion (Guangliang et al., 2017), taking the intestinal phase by study its effect in 3T3-L1 cell line. The 3T3-L1 cell line differentiation process was according by the ATCC. For the evaluation of adipogenesis, the Oil Red O stain kit (Lipid Stain) was used (Oseguera Toledo et al., 2016) (n = 3). Results: The protein percentual was a) 81.55+0.16 % and b) 82.32+0.77%. The in vitro digestibility of protein isolates was a) 93+0.09% and b) 94+0.23%. The 3T3-L1 cell line cultivated with a) and b) obtaining a significant difference (p