Biomolecular of Clear Kefir Probiotics Supplementation as Antidiabetic

Backgroud/Aims: Free radicals may affect hyperglycemia, increase lipid peroxidation, reduced antioxidants and immune response. This study analyzed the effect of clear kefir supplementation on immune response, lipid peroxidation and antioxidant status of hyperglycemia of Type 2 Diabetes Mellitus (T2DM) Patients in Bandung, Indonesia. Methods: The randomized control trial was conducted in 108 T2DM patients. Subjects were allocated randomly into: (1) T2DM with HbA1c 7, given clear kefir 200 ml, (3) T2DM with standar diet. The intervention was runned for 30 days. Lipid peroxidation measured by spectrophotometry. TNFα, IL10 measured by ELISA. SOD and GPX Antioxidants measured by ELISA. Catalase measured by spectrophotometry. Clear kefir characterization was done by microbiology identification. Data were analyzed by One Way ANOVA and Post Hoc Duncan test with significance level p < 0.05. The study was approved by ethic committee. Results: Clear kefir supplementation affected on reduction of MDA, increased IL10, antioxidants status. Statistical showed there were respectively increased of IL10 (p = 0.001), decreased delta of TNFα (p = 0.001) and delta of MDA (p < 0.001). SOD level was increased (p < 0.015). GPx and CAT were also inceased (p < 0.001), except in control groups. Probiotics kefir found as many as 10f–109 cfu/mL and declined to 10e as the decrease in pH during storage. Probiotics were detected, such as: Lactobacillus Sp, Lactococcus and Acetobacter and Saccharomyces Sp. Kefir supplementation decreased the level of MDA, increased of antioxidants and immune response.