Polymorphism of prolactine genes and its association with body weight in Bayang ducks, local duck from West Sumatera, Indonesia

This study aims to determine the polymorphism of Prolactin (PRL | XbaI and PRL | DraI) genes in Bayang ducks using the PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) technique and its association with weight of ducks aged 1-10 weeks. This study used 200 Bayang duck blood samples consisting of 102 male ducks (♂) and 98 female ducks (♀). DNA from blood samples was isolated using the Genomic DNA Purifification Kit (Promega) using protocol from the manucfacture. The DNA was then amplified using two primers with F: 5′-AAA TTC CCT CTC ACA GTT ACA-3′ and R: 5′-GAT GCA GAG ACA AGT TTC ACC-3′ and F: 5′-GAATAGAACACTTGACCCTG-3′ and R: 5′-TAGAGGAGGCAAGCATAG-3′ which produces fragments with a length of 416 bp and 566 bp. Restriction with XbaI enzymes that recognized the binding site (5-TT CTAGA-3′) resulted 3 genotypes: homozygote (+/+), heterozygote (+/−) and homozygote (−/−) with frequencies 0.455, 0.495 and 0.050 respectively and with frequency allel (+) 0.702 and frequency allel (−) 0.297. While the results of the restriction with enzyme DraI found three types of genotypes, namely (+/+), (+/−) and (−/−) with frequencies of 0.64, 0.35 and 0.01 respectively with frequency allel (+) 0.82 and allele frequencies (−) 0.18. From the results of the analysis, it was found that there was no relationship between these two diversity and weight ducks of duck.