Cold cream combination of Garcinia mangostana L. Anredera cordifolia (Ten.) and Centella asiatica extracts on Burn Healing Activity Test

Background: Mangosteen peel (Garcinia mangostana)L., binahong leaf (Anredera cordifolia (Ten.)), and centella herb (Centella asiatica) have been shown to have activity in each phase of burn healing process. Formulating these three plants extract into cold cream preparations is expected to increase the healing activity of burns. Objective: This study aimed to prove the efficacy of the cold cream combination of these plants by examining in vivo burn healing activity using a deep second-degree burn rat model. Materials and Methods: Rat were divided into nine experimental groups (normal, saline solution, silver sulfadiazine, cold cream base, each extract, combination of three extracts, and coldcream combination of three extracts). The burn activity was assessed by inflammatory cell, agiogenesis and collagen formation through histopathological examination. Results: Cold cream combination of mangosteen peel, binahong leaf, and centella herb enhanced the burn wound healing as indicated by progressive improvement in wound healing during 21 days after treatment. Histological result showed that coldcream combination group can reduced inflammatory cell infiltration and increased collagen significantly (p ≤ 0.05), in the other hand same as normal control, but has no effect on angiogenesis. Conclusion: Based on these results, cold cream combination of mangosteen peel, binahong leaf, and centella herb enhanced has a potential effect as an burn healing agent.