SRAP Markers - Based Genetic Biodiversity and Differentiation of Three cultured Goldfish Strains

The genetic biodiversity and differentiation of three representative goldfish species in Beijing were studied using SRAP molecular markers. A total of 122 sites were amplified from 10 primer combinations with good polymorphism, including 99 polymorphic sites. The average polymorphism rate was 80.47% and the average polymorphism information content was 0.264. Diversity indexes of Nei's genes in short-tailed Bubble-eye goldfish, Redhead goldfish and Black Dragon-eye goldfish were 0.225, 0.208 and 0.238, respectively. The Shannon information index of the three groups was 0.342, 0.299 and 0.363, respectively. The proportions of polymorphic loci in the three populations were 69%, 65% and 72%, respectively. The genetic distance indicated that genetic divergence existed between the three groups. The genetic relationship between Bubble-eye goldfish and Redhead goldfish was far, while Redhead goldfish and Black Dragon-eye goldfish was close. This study is of great importance for the research of molecular marker-assisted biodiversity conservation of goldfish.