The Effect of Cryopreservation Method to the Quality of Bovine Cumulus Cells Primary Culture
Cumulus cells are play a role as nucleus donor in Somatic Cell Nuclear Transfer (SCNT) technology. This study was conducted to find a suitable method for cryopreserving cumulus cells in aim to provide donor cell stock of primary cell culture. The freezing temperature was made at three levels: -20°C,...
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Veröffentlicht in: | IOP conference series. Earth and environmental science 2020-04, Vol.478 (1), p.12012 |
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Sprache: | eng |
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Zusammenfassung: | Cumulus cells are play a role as nucleus donor in Somatic Cell Nuclear Transfer (SCNT) technology. This study was conducted to find a suitable method for cryopreserving cumulus cells in aim to provide donor cell stock of primary cell culture. The freezing temperature was made at three levels: -20°C, -80 °C, and -196 °C (liquid nitrogen), while cryopreservation was performed by adding cryoprotectants (CPs) that made from 10% Ethylene Glycol (EG) or 10% Dimethyl Sulfoxide (DMSO) in Dulbecco's Phosphate Buffer Saline (DPBS) + 20% New Born Calf Serum (NBCS) medium. The results showed that the freezing temperature, cryoprotectants and their interaction had no significant effects on the cell concentration (p>0.05). The cell viability efficiency was decreased significantly when the cell were freezing at -20°C compared to freezing temperature at -80°C and -196°C (p |
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ISSN: | 1755-1307 1755-1315 |
DOI: | 10.1088/1755-1315/478/1/012012 |