Silver birch (Betula pendula Roth.) culture initiation in vitro and genotype determined differences in micropropagation

Micropropagation has several advantages over conventional vegetative propagation methods, but it is limited by genotype responsiveness. We assessed the effect of age of the mother-tree and the time of explant collection on culture initiation, as well as the multiplication ability and effect of different nutrient media and plant growth regulators on silver birch genotypes. Explants collected from 1‐year‐old trees (66%) and explants collected in spring (64–67%) developed a significantly (both p