000193: DNA PLOIDY ANALYSIS AND THINPREP CYTOLOGY ARE METHODS OF COMBINED CORRELATION OF THE FINAL DIAGNOSIS IN A CASE OF EPITHELIAL OVARIAN CANCER

Objective: The aim of our study is combined correlation of the final diagnosis with DNA ploidy and ThinPrep method and amelioration of the diagnostic accuracy in a case of malignant epithelial ovarian tumors. Methods: Our study was carried out on 105 patients with malignant epithelial tumors of the ovary. The age of our patients ranged from 22 to 70 years. (Mean value 45.2, SD = 18, 35). For ThinPrep cytology two smears (1 stained by Papanikolaou and 1 by Feulgen) were prepared. In the Feulgan stained smears DNA ploidy measurements were performed using SAMBA 2004 image analyzer. Results: The 60, 95% of the cases of our materials were aneuploid and the remaining 39, 04% euploid. The results detect diploid DNA in 20 from 84 cases of IIC-IV stage (23, 80%). In 4 cases with false negative cytological results aneuploid DNA was detected in the histogram of static cytometry. A statistically significant difference was observed between the final cytological diagnosis and the ploidy status for discrimination of malignant from mesothelial cell (x2 = 11, 25, p < 0,001). The sensitivity, specificity, predictive value of positive result, predictive value of negative result and the diagnostic accuracy of the final cytological diagnosis and DNA ploidy analysis were 97,62%, 100%,100%,91,3% and 98,09% respectively Conclusion: DNA ploidy can not be use as independent diagnostic criteria and only combined DNA ploidy analysis and ThinPrep methods show effective increase of diagnostic accuracy, can be use for the final diagnosis correlation and determinate of prognosis.