000458: THE ROLE OF HPV 16/18 INFECTION OF THE UTERINE CERVIX IN THE ACCURACY OF PREOPERATIVE DETERMINATION OF CERVICAL INTRAEPITHELIAL NEOPLASIA (CIN) SEVERITY

Introduction: The aim of the study was to evaluate the role of infection of the uterine cervix with human papilloma virus (HPV) 16/18 in the preoperative determination of cervical intraepithelial neoplasia (CIN) severity. Patients and Methods: This study included 318 patients with CIN treated with conization. Before surgery, cervical smears were obtained from all patients by using a cytobrush for HPV 16/18 determination. In each patient the preoperative grade of CIN was compared to the definitive grade of CIN after conization with respect to HPV 16/18 infection of the uterine cervix. Results: HPV 16/18 had infected 47.5% of patients with CIN. HPV infection was present in 36.0% of patients with low grade CIN and in 48.5% of patients with high grade CIN (CIN 2, CIN 3, microinva- sive carcinoma), but the difference between both groups was not statistically significant (chi-square test = 0.978; p > 0.05). After conization a higher grade of CIN than on preoperative biopsy was found in 72 patients (22.6%). Among them 30 were HPV 16/18 positive and 42 HPV 16/18 negative. A lower grade of CIN after conization was found in 35 patients, among them were 13 HPV 16/18 positive and 22 HPV 16/18 negative. There was no statistically significant difference between both groups of patients (chi-square test = 0.0565; p. 0.05). Conclusion: The results of this study show that infection with HPV 16/18 has no impact on the accuracy of preoperative determination of CIN severity.