A Novel Comparison of Virulence Genes, Biofilm-Forming Capacity, Antibiotic Resistance, and Level of Reactive Oxygen Species of Sediment, Sewage, and O157 E. coli

Even though sediment Escherichia coli is a common source of contamination in freshwater environments, their characteristics such as virulence genes, biofilm forming capacity, antibiotic resistance, and level of reactive oxygen species (ROS) are not well understood. This study examined E. coli from freshwater sediments, sewage, and a collection of O157 isolates. Multiplex PCR analysis revealed that none of the sediment and 3% of the sewage isolates contained diarrheagenic E. coli genes. However, 12.5% of sediment and 42.4% of sewage isolates contained one or more uropathogenic genes. Biofilm assays determined that sediment E. coli were significantly better biofilm formers ( p