The study of antibacterial, fungicidal and cytotoxic properties of antagonist microorganisms

Microorganisms isolated from natural sources that have antagonistic properties are the objects of the research in this paper. The main objective of the study was to screen and characterize the antagonistic properties of microorganisms isolated from natural sources in connection with the creation of new pharmaceutical substances. Methods of cultivating strains of microorganisms, chromatographic methods, spectrometric, electrophoretic methods of analysis, and methods of cryopreservation of accumulative cultures were applied. In the course of the experiment, nutrient media for cultivating strains of antagonist microorganisms were optimized, and the physiological and biochemical properties of lactic acid bacteria and other antagonist microorganisms were studied. It is proved that lactic acid bacteria and other antagonist microorganisms show antimicrobial properties on a solid medium. The antibiotic resistance of lactic acid bacteria and other antagonist microorganisms was studied, the biocompatibility of lactic acid bacteria and other antagonist microorganisms was proved. During the investigation, the antibacterial properties of the isolated peptide fractions were determined, the fungicidal properties of the isolated peptide fractions were established, and low-molecular protein compounds with antibacterial and antifungicidal activity were identified; the toxicity indicators of identified bacteriocins and fungicides were studied in vitro. The novelty of this work consists in the development of scientifically based approaches to screening and characterization of antagonistic properties of microorganisms isolated from natural sources, as well as to the cultivation of antagonist microorganisms in connection with the creation of new pharmaceutical substances of antimicrobial action.