Prevalence of enterotoxemia and antibiogram of Clostridium perfringens isolated from diarrheic goat in the vicinity of district Tharparkar, Sindh, Pakistan

Goat is amongst the earliest small ruminant species to be domesticated and have been reared for meat and milk purpose. Enterotoxemia is a bacterial disease, also said to be pulpy kidney disease or over-eating disease, occurs in sheep and goat throughout the world. In Caprine this disease is caused by a bacteria called Clostridium perfringens type D, which is a gram positive, spore forming, anaerobic, and rod shaped. 100 faecal samples were obtained from diarrheic goats from four Taluka of District Tharparker Sindh, Pakistan. These samples were collected aseptically in ultra violet treated contamination free polyethylene bags which were contained double volume of phosphate buffer saline (PBS) and samples were brought to the laboratory (Sindh Poultry Vaccine Centre Korangi, Karachi, Sindh, Pakistan), in portable cooler containing an ice pack, then samples were refrigerated at 4C until being processed. A chain of laboratory techniques, including colony characteristics, biochemical analysis, gram staining and growth on Robertson cooked meat (RCM) media was performed. Furthermore, all positive samples were subjected to check their antibiogram through 2-fold Micro Broth Dilution Method by using Minimum Inhibitory Concentration (MIC) method. Among 100 diarrheic samples the percentage of positive cases was (44.44%) in Talulka Dahali followed by Chachro (26.67%), Islamkot (17.78%) and Mithi (11.11%). Among 45 samples, a total of 01 sample was shown their resistant at the concentration of 16μg/ml against Amoxicillin. Although for Chloramphenicol, a total of 01 sample was shown their resistant at the concentration of 16μg/ml and 01 samples was shown their resistant at 32μg/ml concentration. Furthermore, there was no any sample show its resistant at the different concentration of Trimethoprim/Sulphamethaxzol used in this study.