Investigation on spectral and biomedical characterization of rhamnolipid from a marine associated bacterium Pseudomonas aeruginosa (DKB1)
Bio-surfactants are a principal group of significant molecules obtained from the microbial sources expressed with distinctive characteristics like biodegradation of hydrocarbons and also have different biomedical properties. The present investigation aims to assess the biomedical properties of synth...
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| Veröffentlicht in: | Archives of microbiology 2021-07, Vol.203 (5), p.2297-2314 |
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| creator | Sanjivkumar, Muthusamy Deivakumari, Murugan Immanuel, Grasian |
| description | Bio-surfactants are a principal group of significant molecules obtained from the microbial sources expressed with distinctive characteristics like biodegradation of hydrocarbons and also have different biomedical properties. The present investigation aims to assess the biomedical properties of synthesized bio-surfactant, rhamnolipid from
Pseudomonas aeruginosa
(DKB1) under in vitro conditions. The candidate bacterium
P. aeruginosa
(DKB1) was isolated from oil-polluted fishing harbors of Kanyakumari coast. Initially, the bio-surfactant production by this candidate strain was confirmed by oil displacement assay, hemolytic assay, drop collapse assay and emulsification index. Further, the production of bio-surfactant was achieved through submerged fermentation process using Bushnell–Haas mineral salts medium supplemented with 2% olive oil. The yield of the bio-surfactant was attained as 2.4 g/l and confirmed as rhamnolipid through blue agar plate assay; further, the extracted rhamnolipid was purified and characterized through standard procedures. In stability studies, the rhamnolipid could withstand up to pH 12, temperature 100 °C and 15% of NaCl concentration. The biomedical application of rhamnolipid (30 μg ml
−1
) was determined by antibacterial, antioxidant and cytotoxic studies. It exhibited a maximum growth inhibition against
Bacillus subtilis
(26 mm) with the MIC value of 8 μg ml
−1
. In antioxidant test, rhamnolipid expressed significant (
P
|
| doi_str_mv | 10.1007/s00203-021-02220-x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2541127156</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2541127156</sourcerecordid><originalsourceid>FETCH-LOGICAL-c375t-67ff8cc91326f7f7d3ed302136721d5b689992273bfc938626b532890ae2fe513</originalsourceid><addsrcrecordid>eNp9UMtu1DAUtVARHQo_wAJZ6gYWAfveie0s6fBo1UqwAImd5Tj21NXEHuykKv0D_hpDBtghXevqyuehcwh5xtkrzph8XRgDhg0DXh8Aa-4ekBVfIzRMwtcjsmLIoFEd4jF5XMoNYxyUUo_IMaJYC0S1Ij8u4q0rU9iaKaRI65S9s1M2O2riQPuQRjcEW097bbKxk8vh_oD1NF-bMaZd2IeB-pxGauhocoiOmlKSDWZyVWNhzSP9VNw8pDFFU6hxed6GmIqhL95envGXT8hDb3bFPT3sE_Ll_bvPm_Pm6uOHi82bq8aibKdGSO-VtR1HEF56OaAbakyOQgIf2l6orusAJPbedqgEiL5FUB0zDrxrOZ6Q00V3n9O3uWbXN2nOsVpqaNecg-StqChYUDanUrLzep9DzfZdc6Z_ta-X9nW11r_b13eV9PwgPfe1tr-UP3VXAC6AUr_i1uV_3v-R_Ql5YJHD</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2541127156</pqid></control><display><type>article</type><title>Investigation on spectral and biomedical characterization of rhamnolipid from a marine associated bacterium Pseudomonas aeruginosa (DKB1)</title><source>SpringerLink Journals - AutoHoldings</source><creator>Sanjivkumar, Muthusamy ; Deivakumari, Murugan ; Immanuel, Grasian</creator><creatorcontrib>Sanjivkumar, Muthusamy ; Deivakumari, Murugan ; Immanuel, Grasian</creatorcontrib><description>Bio-surfactants are a principal group of significant molecules obtained from the microbial sources expressed with distinctive characteristics like biodegradation of hydrocarbons and also have different biomedical properties. The present investigation aims to assess the biomedical properties of synthesized bio-surfactant, rhamnolipid from
Pseudomonas aeruginosa
(DKB1) under in vitro conditions. The candidate bacterium
P. aeruginosa
(DKB1) was isolated from oil-polluted fishing harbors of Kanyakumari coast. Initially, the bio-surfactant production by this candidate strain was confirmed by oil displacement assay, hemolytic assay, drop collapse assay and emulsification index. Further, the production of bio-surfactant was achieved through submerged fermentation process using Bushnell–Haas mineral salts medium supplemented with 2% olive oil. The yield of the bio-surfactant was attained as 2.4 g/l and confirmed as rhamnolipid through blue agar plate assay; further, the extracted rhamnolipid was purified and characterized through standard procedures. In stability studies, the rhamnolipid could withstand up to pH 12, temperature 100 °C and 15% of NaCl concentration. The biomedical application of rhamnolipid (30 μg ml
−1
) was determined by antibacterial, antioxidant and cytotoxic studies. It exhibited a maximum growth inhibition against
Bacillus subtilis
(26 mm) with the MIC value of 8 μg ml
−1
. In antioxidant test, rhamnolipid expressed significant (
P
< 0.0001) inhibition of total reducing power (44.11%), DPPH activity (61.60%), hydroxyl radical (83.30%) and nitric oxide (51.86%) scavenging ability at 100 μg ml
−1
with the respective IC50 values of 130.50, 77.18, 52.08 and 95.43 μg ml
−1
. The anticancer activity of the rhamnolipid was assessed with the help of MTT test against MCF-7, HT-29 and E-143 cancer cell lines individually, and the viability of the cells was observed, respectively, as 10.24, 17.66 and 13.50% at 250 μg ml
−1
concentration with the respective IC50 values of 140.2, 81.02 and 138.9 μg ml
−1
. From the results, it could be concluded that the rhamnolipid produced by
P. aeruginosa
(DKB1) isolated from oil-polluted area has effective biomedical properties.</description><identifier>ISSN: 0302-8933</identifier><identifier>EISSN: 1432-072X</identifier><identifier>DOI: 10.1007/s00203-021-02220-x</identifier><identifier>PMID: 33646338</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Anticancer properties ; Antiinfectives and antibacterials ; Antioxidants ; Antitumor activity ; Assaying ; Bacteria ; Biochemistry ; Biodegradation ; Biomedical and Life Sciences ; Biomedical materials ; Biotechnology ; Cell Biology ; Cytotoxicity ; Ecology ; Emulsification ; Fermentation ; Harbors ; Hydroxyl radicals ; Life Sciences ; Microbial Ecology ; Microbiology ; Microorganisms ; Minimum inhibitory concentration ; Nitric oxide ; Oil pollution ; Olive oil ; Original Paper ; Pollutants ; Pseudomonas aeruginosa ; Rhamnolipids ; Salts ; Scavenging ; Sodium chloride ; Surfactants ; Tumor cell lines</subject><ispartof>Archives of microbiology, 2021-07, Vol.203 (5), p.2297-2314</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature 2021</rights><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-67ff8cc91326f7f7d3ed302136721d5b689992273bfc938626b532890ae2fe513</citedby><cites>FETCH-LOGICAL-c375t-67ff8cc91326f7f7d3ed302136721d5b689992273bfc938626b532890ae2fe513</cites><orcidid>0000-0002-2747-4113</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00203-021-02220-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00203-021-02220-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33646338$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sanjivkumar, Muthusamy</creatorcontrib><creatorcontrib>Deivakumari, Murugan</creatorcontrib><creatorcontrib>Immanuel, Grasian</creatorcontrib><title>Investigation on spectral and biomedical characterization of rhamnolipid from a marine associated bacterium Pseudomonas aeruginosa (DKB1)</title><title>Archives of microbiology</title><addtitle>Arch Microbiol</addtitle><addtitle>Arch Microbiol</addtitle><description>Bio-surfactants are a principal group of significant molecules obtained from the microbial sources expressed with distinctive characteristics like biodegradation of hydrocarbons and also have different biomedical properties. The present investigation aims to assess the biomedical properties of synthesized bio-surfactant, rhamnolipid from
Pseudomonas aeruginosa
(DKB1) under in vitro conditions. The candidate bacterium
P. aeruginosa
(DKB1) was isolated from oil-polluted fishing harbors of Kanyakumari coast. Initially, the bio-surfactant production by this candidate strain was confirmed by oil displacement assay, hemolytic assay, drop collapse assay and emulsification index. Further, the production of bio-surfactant was achieved through submerged fermentation process using Bushnell–Haas mineral salts medium supplemented with 2% olive oil. The yield of the bio-surfactant was attained as 2.4 g/l and confirmed as rhamnolipid through blue agar plate assay; further, the extracted rhamnolipid was purified and characterized through standard procedures. In stability studies, the rhamnolipid could withstand up to pH 12, temperature 100 °C and 15% of NaCl concentration. The biomedical application of rhamnolipid (30 μg ml
−1
) was determined by antibacterial, antioxidant and cytotoxic studies. It exhibited a maximum growth inhibition against
Bacillus subtilis
(26 mm) with the MIC value of 8 μg ml
−1
. In antioxidant test, rhamnolipid expressed significant (
P
< 0.0001) inhibition of total reducing power (44.11%), DPPH activity (61.60%), hydroxyl radical (83.30%) and nitric oxide (51.86%) scavenging ability at 100 μg ml
−1
with the respective IC50 values of 130.50, 77.18, 52.08 and 95.43 μg ml
−1
. The anticancer activity of the rhamnolipid was assessed with the help of MTT test against MCF-7, HT-29 and E-143 cancer cell lines individually, and the viability of the cells was observed, respectively, as 10.24, 17.66 and 13.50% at 250 μg ml
−1
concentration with the respective IC50 values of 140.2, 81.02 and 138.9 μg ml
−1
. From the results, it could be concluded that the rhamnolipid produced by
P. aeruginosa
(DKB1) isolated from oil-polluted area has effective biomedical properties.</description><subject>Anticancer properties</subject><subject>Antiinfectives and antibacterials</subject><subject>Antioxidants</subject><subject>Antitumor activity</subject><subject>Assaying</subject><subject>Bacteria</subject><subject>Biochemistry</subject><subject>Biodegradation</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical materials</subject><subject>Biotechnology</subject><subject>Cell Biology</subject><subject>Cytotoxicity</subject><subject>Ecology</subject><subject>Emulsification</subject><subject>Fermentation</subject><subject>Harbors</subject><subject>Hydroxyl radicals</subject><subject>Life Sciences</subject><subject>Microbial Ecology</subject><subject>Microbiology</subject><subject>Microorganisms</subject><subject>Minimum inhibitory concentration</subject><subject>Nitric oxide</subject><subject>Oil pollution</subject><subject>Olive oil</subject><subject>Original Paper</subject><subject>Pollutants</subject><subject>Pseudomonas aeruginosa</subject><subject>Rhamnolipids</subject><subject>Salts</subject><subject>Scavenging</subject><subject>Sodium chloride</subject><subject>Surfactants</subject><subject>Tumor cell lines</subject><issn>0302-8933</issn><issn>1432-072X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9UMtu1DAUtVARHQo_wAJZ6gYWAfveie0s6fBo1UqwAImd5Tj21NXEHuykKv0D_hpDBtghXevqyuehcwh5xtkrzph8XRgDhg0DXh8Aa-4ekBVfIzRMwtcjsmLIoFEd4jF5XMoNYxyUUo_IMaJYC0S1Ij8u4q0rU9iaKaRI65S9s1M2O2riQPuQRjcEW097bbKxk8vh_oD1NF-bMaZd2IeB-pxGauhocoiOmlKSDWZyVWNhzSP9VNw8pDFFU6hxed6GmIqhL95envGXT8hDb3bFPT3sE_Ll_bvPm_Pm6uOHi82bq8aibKdGSO-VtR1HEF56OaAbakyOQgIf2l6orusAJPbedqgEiL5FUB0zDrxrOZ6Q00V3n9O3uWbXN2nOsVpqaNecg-StqChYUDanUrLzep9DzfZdc6Z_ta-X9nW11r_b13eV9PwgPfe1tr-UP3VXAC6AUr_i1uV_3v-R_Ql5YJHD</recordid><startdate>20210701</startdate><enddate>20210701</enddate><creator>Sanjivkumar, Muthusamy</creator><creator>Deivakumari, Murugan</creator><creator>Immanuel, Grasian</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><orcidid>https://orcid.org/0000-0002-2747-4113</orcidid></search><sort><creationdate>20210701</creationdate><title>Investigation on spectral and biomedical characterization of rhamnolipid from a marine associated bacterium Pseudomonas aeruginosa (DKB1)</title><author>Sanjivkumar, Muthusamy ; Deivakumari, Murugan ; Immanuel, Grasian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-67ff8cc91326f7f7d3ed302136721d5b689992273bfc938626b532890ae2fe513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Anticancer properties</topic><topic>Antiinfectives and antibacterials</topic><topic>Antioxidants</topic><topic>Antitumor activity</topic><topic>Assaying</topic><topic>Bacteria</topic><topic>Biochemistry</topic><topic>Biodegradation</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedical materials</topic><topic>Biotechnology</topic><topic>Cell Biology</topic><topic>Cytotoxicity</topic><topic>Ecology</topic><topic>Emulsification</topic><topic>Fermentation</topic><topic>Harbors</topic><topic>Hydroxyl radicals</topic><topic>Life Sciences</topic><topic>Microbial Ecology</topic><topic>Microbiology</topic><topic>Microorganisms</topic><topic>Minimum inhibitory concentration</topic><topic>Nitric oxide</topic><topic>Oil pollution</topic><topic>Olive oil</topic><topic>Original Paper</topic><topic>Pollutants</topic><topic>Pseudomonas aeruginosa</topic><topic>Rhamnolipids</topic><topic>Salts</topic><topic>Scavenging</topic><topic>Sodium chloride</topic><topic>Surfactants</topic><topic>Tumor cell lines</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sanjivkumar, Muthusamy</creatorcontrib><creatorcontrib>Deivakumari, Murugan</creatorcontrib><creatorcontrib>Immanuel, Grasian</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Virology and AIDS Abstracts</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><jtitle>Archives of microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sanjivkumar, Muthusamy</au><au>Deivakumari, Murugan</au><au>Immanuel, Grasian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Investigation on spectral and biomedical characterization of rhamnolipid from a marine associated bacterium Pseudomonas aeruginosa (DKB1)</atitle><jtitle>Archives of microbiology</jtitle><stitle>Arch Microbiol</stitle><addtitle>Arch Microbiol</addtitle><date>2021-07-01</date><risdate>2021</risdate><volume>203</volume><issue>5</issue><spage>2297</spage><epage>2314</epage><pages>2297-2314</pages><issn>0302-8933</issn><eissn>1432-072X</eissn><abstract>Bio-surfactants are a principal group of significant molecules obtained from the microbial sources expressed with distinctive characteristics like biodegradation of hydrocarbons and also have different biomedical properties. The present investigation aims to assess the biomedical properties of synthesized bio-surfactant, rhamnolipid from
Pseudomonas aeruginosa
(DKB1) under in vitro conditions. The candidate bacterium
P. aeruginosa
(DKB1) was isolated from oil-polluted fishing harbors of Kanyakumari coast. Initially, the bio-surfactant production by this candidate strain was confirmed by oil displacement assay, hemolytic assay, drop collapse assay and emulsification index. Further, the production of bio-surfactant was achieved through submerged fermentation process using Bushnell–Haas mineral salts medium supplemented with 2% olive oil. The yield of the bio-surfactant was attained as 2.4 g/l and confirmed as rhamnolipid through blue agar plate assay; further, the extracted rhamnolipid was purified and characterized through standard procedures. In stability studies, the rhamnolipid could withstand up to pH 12, temperature 100 °C and 15% of NaCl concentration. The biomedical application of rhamnolipid (30 μg ml
−1
) was determined by antibacterial, antioxidant and cytotoxic studies. It exhibited a maximum growth inhibition against
Bacillus subtilis
(26 mm) with the MIC value of 8 μg ml
−1
. In antioxidant test, rhamnolipid expressed significant (
P
< 0.0001) inhibition of total reducing power (44.11%), DPPH activity (61.60%), hydroxyl radical (83.30%) and nitric oxide (51.86%) scavenging ability at 100 μg ml
−1
with the respective IC50 values of 130.50, 77.18, 52.08 and 95.43 μg ml
−1
. The anticancer activity of the rhamnolipid was assessed with the help of MTT test against MCF-7, HT-29 and E-143 cancer cell lines individually, and the viability of the cells was observed, respectively, as 10.24, 17.66 and 13.50% at 250 μg ml
−1
concentration with the respective IC50 values of 140.2, 81.02 and 138.9 μg ml
−1
. From the results, it could be concluded that the rhamnolipid produced by
P. aeruginosa
(DKB1) isolated from oil-polluted area has effective biomedical properties.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>33646338</pmid><doi>10.1007/s00203-021-02220-x</doi><tpages>18</tpages><orcidid>https://orcid.org/0000-0002-2747-4113</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0302-8933 |
| ispartof | Archives of microbiology, 2021-07, Vol.203 (5), p.2297-2314 |
| issn | 0302-8933 1432-072X |
| language | eng |
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| source | SpringerLink Journals - AutoHoldings |
| subjects | Anticancer properties Antiinfectives and antibacterials Antioxidants Antitumor activity Assaying Bacteria Biochemistry Biodegradation Biomedical and Life Sciences Biomedical materials Biotechnology Cell Biology Cytotoxicity Ecology Emulsification Fermentation Harbors Hydroxyl radicals Life Sciences Microbial Ecology Microbiology Microorganisms Minimum inhibitory concentration Nitric oxide Oil pollution Olive oil Original Paper Pollutants Pseudomonas aeruginosa Rhamnolipids Salts Scavenging Sodium chloride Surfactants Tumor cell lines |
| title | Investigation on spectral and biomedical characterization of rhamnolipid from a marine associated bacterium Pseudomonas aeruginosa (DKB1) |
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