Nanoparticle tracking analysis as a process analytical tool for characterising magnetosome preparations

[Display omitted] •Nanoparticle Tracking Analysis (NTA) allowed measurement of magnetosome concentration and size.•NTA detected particles in TEM size range of single isolated magnetosomes in polydisperse samples.•NTA and TEM magnetosome sizing data showed striking correspondence at low ultrasonication times.•NTA was able to detect dynamic changes in the aggregation state of magnetosomes in solution.•NTA could become a powerful PAT/QC tool in magnetosome research, development and manufacture. Nanoparticle Tracking Analysis (NTA) has been employed to measure the particle concentration and size distribution of magnetosomes extracted and purified from Magnetospirillum gryphiswaldense MSR-1, and then exposed to probe ultrasonication for various times, or 1% (w/v) sodium dodecyl sulphate (SDS) for 1 h. Particle concentration increased 3.7-fold over the first 15 min of ultrasonication (from 2 × 108 to >7.3 × 108 particles mL−1), but fell steeply to ∼3.6 × 108 particles mL−1 after 20 min. NTA of untreated magnetosome preparation confirmed a wide particle distribution dominated by larger species (D[1,0] = 312 nm; Dn50 = 261 nm; mode = 243 nm) with no particles in the size range of isolated single magnetosomes. After 5 min of ultrasonication the whole particle size distribution shifted to smaller size (D[1,0] = 133 nm; Dn50 = 99 nm; mode = 36 nm, corresponding to individual magnetosomes), but longer treatment times (15 and 20 min) reversed the previous transition; all characteristic numbers of the particle size distributions increased and very few small particles were detected. Side-by-side comparison of NTA and TEM sizing data revealed remarkable similarity at low ultrasonication times, with both showing single magnetosomes accounted for ∼30% population after 5 min. Exposure of magnetosomes to SDS resulted in a ∼3-fold increase in particle concentration to 5.8 × 108 particles mL−1, narrowing of the size distribution and gross elimination of particles below 60 nm. We conclude that NTA is a rapid cost-effective technique for measuring particle concentration, size distribution and aggregation state of magnetosomes in solution.