Experimental evaluation of anticonvulsant activity of calcium channel blocker - cinnarizine alone and in combination with N-methyl-D-aspartate receptor antagonist ketamine in mice

Aim and Objectives: The aim of the study was to evaluate the anticonvulsant activity of calcium channel blocker, using cinnarizine alone and in combination with NMDA receptor antagonist ketamine by producing pentylenetetrazol (PTZ)-induced convulsions in male albino mice. The mean time of onset of convulsion (seconds) mean duration of convulsion, and percentage of protection readings were recorded for 1 h. Results: Group 1 (Control group) displayed 0% protection from convulsions and Group 2 (Standard group) exhibited 100% and Group 5 (Test group 3) (T3) exhibited 66.66% protection from convulsions, indicating that the combination of cinnarizine (30 mg/kg) and ketamine (50mg/kg) was effective in PTZ-induced seizures in male albino mice. The objective of the study is to evaluate the anticonvulsant activity of calcium channel blocker, using cinnarizine alone and in combination with NMDA receptor antagonist ketamine by producing PTZ induced convulsions in male Albino mice. Based on the bodyweight of mice, the dose was calculated and administered subcutaneously in the scruff of the mice's neck [Figure 1], The majority of the animals showed a short clonic forelimb flexion response followed by a tonic hindlimb extension response [Figure 2], Standard Drug Administration Sodium valproate It was used as a standard drug and was injected at the dose of 150 mg/kg body wt, intraperitoneally 30 min before the induction of seizures.