Developed cloud point extraction coupled with onium system for separation and determination cobalt in Biological samples

In many spectrophotometric studies coupled onium system with CPE to become more selective and sensitive method for many ions of Hg (II), Fe (III) in addition to Co (II) use N-benzoyl-L-arginine ethyl ester hydrochloride (BAEE) to form ion pair association give lower detection limit D. L= 0.023 ppm, 0.210 ppm, 1.2731·10-5mol /L respectively12,13. C.General Method: Aqueous solutions (10 mL) in volume contain optimal quantity of Co (II) and optimal concentration of HCl, (1M0-4 M) of MS in the existence suitable volume of non-ionic surfactant TritonX-100. A.Effect of Hydrochloric Acid Concentration: Prepare aqueous solutions 10 mL in volume contain 100 pg Co (II) of and rising concentration of hydrochloric acid HCl in existence 0.5 mL Triton X-100 and (1M0-4 M) MS. Heat solutions to an appropriate temperature and time up to forming CPL. The results show enhancement in extraction efficiency with existence electrolyte in aqueous solution because electrolyte effect to decrease dielectric constant and polarity of aqueous solution and this help to destroy hydration shell of Co (II) and increase formation of onium species so that increase dehydration and all that effect to increase concentration of onium species formation and extraction15,29.