Development and Validation of spectrophotometric estimation of vildagliptin through Oxidative coupling reaction using MBTH reagent in Pharmaceutical dosage form

Vildagliptin react with 3-methyl-2benzothiazolinone hydrazone hydrochloride reagent and ferric chloride to form green colored chromogen.3 MBTH react with aldehyde first to form an azine, remaining MBTH, it is oxidized to another species which combines with the azine to form formazan. so, if there is enough aldehyde, all the MBTH is converted to azine and there is no formation of blue color. [...]by using the limiting agent MBTH to test the amount of aldehyde around the point of interest, then less aldehyde would produce more blue color and more aldehyde would produce less blue color. MBTH reagent obtained from Sisco Research laboratories pvt.ltd, Ferric chloride anhydrous obtained from Dev enterprise, Hydrochloric acid obtained from Umiya chemical industry. and all other chemicals were of analytical grade. The optimum conditions such as Beer's law limits, molar absorptivity and other regression characteristics like slope (m), intercept (c), correlation coefficient were calculated. An accurately weighed of powder equivalent to 100mg of Vildagliptin was dissolved in a 100ml of methanol and mixed for about 5 min and then filtered. 10ml of aliquots was pipetted out into 100ml volumetric flask and the volume was made up to the mark with methanol to obtain the final concentration of 100pg/ml (Stock solution).