Ginkgolide B derivative synthesis and their effects on the viability of SKOV3 cells

The natural product Ginkgolide B was used as a raw material and modified by esterification on C10-OH or C1-OH to obtain 11 derivatives ( 1 – 11 ), which were structurally characterized with nuclear magnetic resonance spectroscopy. An MTT assay-based in vitro tumor proliferation inhibitory activity test showed that compounds 2 , 3 , 6 , 7 , 10 , and 11 exhibited strong inhibitory activity against the human ovarian cancer cells SKOV3, with IC 50 values of 16.05 µmol/L, 15.65 µmol/L, 32.00 µmol/L, 63.30 µmol/L, 23.20 µmol/L, and 31.10 µmol/L, respectively. Annexin V/PI double staining assay showed that compound 2 induced apoptosis in SKOV3 cells to a slightly greater extent than GB and compounds 5 and 9 , with an apoptosis rate of 31.68%.