The Effect of Starting Shocking Time on Carbon, Nitrogen, and Organic Matter Absorption of Bacillus megaterium BM1 in Vegetative and Sporulation Phases

The application of shock induction is concerned with the sporulation. The essential indicator of the vegetative cell and spore quantities is influenced by the absorption of the organic compound components. The principal objective of this experiment was to establish the impact of starting shocking time on organic carbon, total nitrogen, and organic matter absorbed by B. megaterium BM1 in vegetative and sporulation phases. This research was conducted in 250 ml Erlenmeyer flasks with 200 ml media and was added with 1% (v/v) bacterial starter (1.7 × 10 8 cells/ml −1 ). The test media were incubated at 37 °C and shocking started at 5, 10, and 15 hours after inoculation, respectively. The sample from each test medium was measured as much as 15 ml to calculate organic compound uptake. The results showed that the carbon and nitrogen concentrations in the culture media are decreasing from the vegetative phase to the sporulation period throughout the given treatments. The highest organic carbon and total nitrogen absorption in vegetative phase occurred at the starting shocking at 10 th hour and 15 th hour with a value of more than 30%. However, in the sporulation, the highest of three parameters happened in the starting shocking time at the 5 th hour.