Dual-signal based immunoassay for colorimetric and photothermal detection of furazolidone

With MnO2 and AuNPs-mediated colorimetric/photothermal nanoparticles as the labeling substrate to improve light-to-heat conversion ability and visible signal, a dual-signal immunoassay biosensor was developed for sensitive and accurate detection of furazolidone. [Display omitted] •A dual-signal immunoassay biosensor was developed for furazolidone detection.•The colorimetric and photothermal signals provided more comprehensive and precise results.•Application of immunoassay in food sample with high sensitivity and selectivity. As a point-of-care testing method, lateral flow immunoassays are attracting extensive attentions for antibiotic residue detections; however, immunoassay techniques for qualitative and quantitative detections are limited by the poor sensitivity and requirement for cumbersome quantitative devices. To overcome these limitations, MnO2-Au, with an excellent carrying capacity of Au nanoparticles and high photothermal signals, was introduced and a dual-signal immunoassay using colorimetric/photothermal MnO2-Au signal probe was proposed for furazolidone (FZD) detection. The dual-signal immunoassay not only enables visual detection relying on color change, but also enables quantitative detection via converting conventional assay signals into heat signals using a thermal infrared imager recording. Based on the excellent light-to-heat conversion activity of the bifunctional MnO2-Au, the FZD metabolite of 3-amino-2-oxazolidinone (AOZ) can be qualitatively detected in virtue of colorimetric signals with a visual limit of 1 ng mL−1 and quantitatively detected by photothermal signals with a detection limit of 0.43 ng mL−1. The dual-signal immunoassay biosensor was well applied in food samples with acceptable recoveries of 80.6–106 %. The immunoassay that integrates colorimetric and photothermal analysis will hold a broader application area in real-time monitoring.