Grafting of Fluorescence-labeled ssDNA onto Inorganic Nanosheets and Detection of a Target DNA

Grafting of Fluorescence-labeled ssDNA onto Inorganic Nanosheets and Detection of a Target DNA

A single stranded DNA (ssDNA) labeled with fluorescein isothiocyanate (FITC) was grafted on hexaniobate nanosheets as confirmed by infrared spectra, X-ray diffraction, and confocal laser scanning microscopy; this is the first case that a ssDNA was grafted on nanosheets. The grafted ssDNA retained th...

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Veröffentlicht in:Chemistry letters 2021, Vol.50 (4), p.632-635
Hauptverfasser: Anraku, Shinya, Kaneko, Yoshiro, Miyamoto, Nobuyoshi
Format: Artikel
Sprache:eng
Schlagworte:
Fluorescein
Fluorescence
Grafting
Infrared spectra
Nanosheets
Scanning microscopy
Target detection
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Zusammenfassung:A single stranded DNA (ssDNA) labeled with fluorescein isothiocyanate (FITC) was grafted on hexaniobate nanosheets as confirmed by infrared spectra, X-ray diffraction, and confocal laser scanning microscopy; this is the first case that a ssDNA was grafted on nanosheets. The grafted ssDNA retained the capability of hybridization with complementary ssDNA to form double stranded DNA so that the fluorescence of FITC was effectively quenched by hybridization with the target ssDNA having a quencher moiety.
ISSN:0366-7022
1348-0715
DOI:10.1246/cl.200698