Hydrogen peroxide concentration measurement by an enzymatic/holographic hybrid structured sensor with polarized spectrometry

[Display omitted] •To develop an enzymatic/holographic hybrid structured sensor to measure H2O2 concentration.•To integrate this sensor into a polarized spectrometer.•Results indicated the measured range of H2O2 could be obtained by horizontally polarized light.•Optimum resolution of proposed method can be better than 0.033 ppm.•An alternated method for ppm level of H2O2 concentration measurement. In order to increase the measuring range and resolution of hydrogen peroxide concentration, fabrication of an enzymatic/holographic hybrid structured hydrogen peroxide sensor and the polarized spectrometry were proposed. The hybrid structured H2O2 sensor consisted of an enzymatic sensing film and a holographic plate. Diffraction gratings recorded by holographic plate provided first order wavelength separation and specificity enzyme reacted with H2O2. Smaller grating pitch of sensor exhibited a narrower wavelength bandwidth selection of light source that can be enhanced stability and resolution of the proposed system. Furthermore, the results showed that the measurement range of the H2O2 concentration would be larger of the measurement with polarized light. The results showed that the measurement range of H2O2 concentration could cover 10−5% to 10-2% with the hybrid structured H2O2 sensor. The results also indicated that use of horizontal polarized light to measure the H2O2 concentration exhibited high measurement sensitivity with value of 8043 (photon counts/%) and the optimum resolution of proposed method can be better than 3.3 × 10-6% (0.033 ppm).