Effect of rootstock and bio-fertilizers on some mineral concentrations in the leaves of Local Lemon (Citrus limon L.) transplants and available nutrients in the Media

The study was conducted in the Lath-house/Department of Horticulture and landscape design/College of Agriculture and Forestry/University of Mosul on budded local lemon ( Citrus limon L.) at one year old, by using Randomized Complete Block Design (RCBD) with two factors, three replications and four transplants for each experimental unit, First factor was rootstock(soure orange and Citrumelo swingle ), second factor was inoculation with Bacillus subtilis bacteria and Trichoderma harzianum fungi, in addition to control treatment (without inoculation), as the inoculation with the bacterial and fungus suspension was carried out twice a season according to the treatments, by adding 10 ml.transplant −1 in each addition, first was in the mid-March and the second was after three months of first treatment. Results indicated that the transplants budded on C. swingle gave the highest values of leaves concentration and soil availability of N, P and available Fe in the media, while the transplants budded on soure orange gave the highest values of leaves K and Fe concentrations. As for biological fertilizers the inoculation with T. harzianum gave the highest values of leaves N and K concentrations and availability of N and Fe in the media, while the inoculation with B. subtilis gave the highest values of other parameters(leaves concentrations of P and Fe and P availability in the media). The interaction between rootstock and bio-fertilizers significantly effected on all studied characteristics, the interaction between C. swingle and T. harzianum gave the highest values of leaves N and available N in the media, while the interaction between C. swingle and B. subtilis gave the highest values of leaves P and its availability in the media, as for leaves K, the interaction between soure orange and T. harzianum gave the highest values of it, while the interaction between soure orange and B. subtilis gave the highest values of leaves Fe concentration.