Hydrolysis optimization of tobacco stems with ultrasonic-assisted hydrolysis method

The development of biofuel in Indonesia is rife to reduce the consumption of fuel oil. Bioethanol has advantages compared to fuel oil, such as lower carbon monoxide emissions making it environmentally friendly. Besides that, from being used as a fuel, bioethanol is used as raw material for alcohol, pharmaceutical, and cosmetics derivatives. Biomass is one of the bioethanol’s raw materials available in Indonesia, one of which is tobacco stem. In this research, the tobacco stem hydrolysis process carried out using the ultrasonic-assisted hydrolysis method. The variable of the hydrolysis process is H 2 SO 4 solution, particle size, and time. The data analysis used Design Expert with a Central Composite Design method. The purpose of this research was to determine the optimum of particle size, H 2 SO 4 concentration, and the time in the hydrolysis process of tobacco stems with the ultrasonic-assisted hydrolysis method. The optimum resulting in reducing sugar of ultrasonic-assisted hydrolysis is 6.921 µg/µL.