Kinetics study of enzymatic hydrolysis of Tetraselmis chuii using Valjamae model

The Tetraselmis chuii microalgae have a potential as an alternative raw material for producing bioethanol, because of its low lignin and high carbohydrate content. The purpose of this research is to study the kinetics of enzymatic hydrolysis of Tetraselmis chuii using Valjamae model. This experiment was carried out with a variety of enzymes (cellulase, xylanase, and cellulase-xylanase mixture, enzyme concentrations (10, 20, and 30% w/w), times (10, 20, 30, and 40 min), and temperatures (40 and 45 °C). The results show that the activation energy of hydrolysis using cellulase-xylanase mixture is 27,253 J/mol, xylanase 116,121J/mol, and cellulose 140,914 J/mol. At 45 °C, the concentration of the cellulase-xylanase mixture increased (10, 20, and 30% w/w), the reaction rate constants (0.028, 0.110, and 0.171 1/min) as well as the fractal exponents (0.4, 0.6, and 0.7). Thus, the glucose production rate is higher, where at 40 minutes produces higher yields (23.5, 25.5, 28.5, and 32.5%). In this study, the constant rate of Tetraselmis chuii hydrolysis using cellulase-xylanase mixture at 45 °C is 0.1714 1/min; higher than the one conducted at 40°C which is 0.1454 1/min. The kinetic parameters of the enzymatic hydrolysis are expected to help in bioreactor design.