Protection of Pegagan (Centella asiatica) Extract Through Hsp60 and Bax Expression on Stunting Model Zebrafish Larvae (Danio rerio) By Rotenone Inducted

Objective: Rotenone is a pesticide (insecticide and fish killers) that is widely used in water. This study utilised rotenone as a model to initiate the occurrence of stunting on zebrafish larvae. Rotenone acts to inhibit the mitochondrial complex I to form an Adenine Tri Phosphate (ATP), Centella asitica(CA) is commonly used as Ayurveda medication as the triterpene in CA’s ingredient and antioxidant protect cells from oxidative damage. This study aimed to know the effect of CA extract on the stunting model zebrafish larvae induced by rotenone through Hsp60 and Bax expression. Methods: Zebrafish embryos were exposed with rotenone [10 ppb] and CA extracts(concentration 2,5 µg/mL; 5 µg/mL and 10 µg/Ml) from the age of 2 to 72 hpf (hour post fertilization).The body length was measured at the age of 3, 6, and 9 dpf (day post fertilization) using Image Raster software. The measurement of Hsp60 and Bax expression used IHC method on wholemount zebrafish larvae through DAB colouring. Density integrated was observed with Image J software. Results: The results of the study showed, There was an increase in Hsp60 and Bax expression on the zebrafish larvae induced by rotenone. In addition, CA extract with concentration [5 µg/mL] increased the body length at the age of 9 dpf (p