Growth of entomopathogenic fungi colonies Metarhizium anisopliae (Metchnikoff) Sorokin enriched with termite juice

Coptotermes curvignathus are termites classified as pest organisms or destructive organisms, because these termites are often found to attack homes, buildings, and plantations. Entomopathogenic fungi of Metarhizium anisopliae is an alternative way to control the termite in environmentally friendly approach. This study aimed to determine the combination media of termite juice concentration for the growth of Metarhizium anisopliae . The fungi were inoculated on growth media (Potato Dextrose Agar) supplemented with termite juice using various concentration of 0% (control), 50%, 75% and 100%. This study showed that fungi grown on media supplemented with 50%, 75 of termite juice produced 2,9 × 10 7 spore/mL density. This result was not significantly different from the media supplemented with 25% and 75% of termite juice yielding 2,4 × 10 7 and 2,7 × 10 7 spore/mL density, respectively. However, this result was significantly different compared to the control treatment and media supplemented with 100% of termite juice yielding spore density of 1,1 × 10 7 and 1,4 × 10 7 , respectively. The growth media supplemented with termite juice is considered to have more nutritional composition to stimulate spore formation promising its application for promoting media of entomopathogenic fungi.